Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway
ABSTRACTLow-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) play a pivotal role by regulating plasma low-density lipoprotein cholesterol (LDL-c) levels. Dihydromyricetin (DMY), the most abundant natural flavonoid in rattan tea, has proven anti-atherogeni...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | CyTA - Journal of Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2023.2252044 |
| _version_ | 1797392455723646976 |
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| author | Li-Tian Wang Dan-Dan Hu Hua-Rong Liu Huai-Liu Yin Rui Mu Yu-Hang Yang Run-Yu Zhao Jun Sheng Ye-Wei Huang Xuan-Jun Wang |
| author_facet | Li-Tian Wang Dan-Dan Hu Hua-Rong Liu Huai-Liu Yin Rui Mu Yu-Hang Yang Run-Yu Zhao Jun Sheng Ye-Wei Huang Xuan-Jun Wang |
| author_sort | Li-Tian Wang |
| collection | DOAJ |
| description | ABSTRACTLow-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) play a pivotal role by regulating plasma low-density lipoprotein cholesterol (LDL-c) levels. Dihydromyricetin (DMY), the most abundant natural flavonoid in rattan tea, has proven anti-atherogenic effects, but the underlying molecular mechanisms remain poorly understood. Therefore, we studied the effects of DMY on LDLR and PCSK9. The results showed DMY promoted LDLR protein and mRNA expression and increased LDL uptake in HepG2 cells. DMY inhibited intracellular PCSK9 protein and mRNA expression. And it also significantly reduced PCSK9 levels in the cell culture medium. Furthermore, DMY inhibited the expression of PCSK9 through the liver nuclear transcription factor 1 A (HNF1A) and increased the protein expression of LDLR. Taken together, our results support the idea that DMY regulates LDL-c metabolism through PCSK9/LDLR signaling. This study reveals the potential mechanism of DMY’s anti-atherogenic effect and provides a theoretical basis for dietary DMY supplementation. |
| first_indexed | 2024-03-08T23:48:40Z |
| format | Article |
| id | doaj.art-3fe7cb1779a54e69b25a333a17240776 |
| institution | Directory Open Access Journal |
| issn | 1947-6337 1947-6345 |
| language | English |
| last_indexed | 2024-03-08T23:48:40Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj.art-3fe7cb1779a54e69b25a333a172407762023-12-13T14:31:46ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452023-12-0121155456010.1080/19476337.2023.2252044Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathwayLi-Tian Wang0Dan-Dan Hu1Hua-Rong Liu2Huai-Liu Yin3Rui Mu4Yu-Hang Yang5Run-Yu Zhao6Jun Sheng7Ye-Wei Huang8Xuan-Jun Wang9Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaABSTRACTLow-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) play a pivotal role by regulating plasma low-density lipoprotein cholesterol (LDL-c) levels. Dihydromyricetin (DMY), the most abundant natural flavonoid in rattan tea, has proven anti-atherogenic effects, but the underlying molecular mechanisms remain poorly understood. Therefore, we studied the effects of DMY on LDLR and PCSK9. The results showed DMY promoted LDLR protein and mRNA expression and increased LDL uptake in HepG2 cells. DMY inhibited intracellular PCSK9 protein and mRNA expression. And it also significantly reduced PCSK9 levels in the cell culture medium. Furthermore, DMY inhibited the expression of PCSK9 through the liver nuclear transcription factor 1 A (HNF1A) and increased the protein expression of LDLR. Taken together, our results support the idea that DMY regulates LDL-c metabolism through PCSK9/LDLR signaling. This study reveals the potential mechanism of DMY’s anti-atherogenic effect and provides a theoretical basis for dietary DMY supplementation.https://www.tandfonline.com/doi/10.1080/19476337.2023.2252044Cholesterol metabolismASCVDHNF1ALDLRPCSK9 |
| spellingShingle | Li-Tian Wang Dan-Dan Hu Hua-Rong Liu Huai-Liu Yin Rui Mu Yu-Hang Yang Run-Yu Zhao Jun Sheng Ye-Wei Huang Xuan-Jun Wang Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway CyTA - Journal of Food Cholesterol metabolism ASCVD HNF1A LDLR PCSK9 |
| title | Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway |
| title_full | Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway |
| title_fullStr | Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway |
| title_full_unstemmed | Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway |
| title_short | Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway |
| title_sort | dihydromyricetin promotes ldl metabolism in hepg2 cells through the pcsk9 ldlr pathway |
| topic | Cholesterol metabolism ASCVD HNF1A LDLR PCSK9 |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2023.2252044 |
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