Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
Abstract Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of s...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-25532-3 |
| _version_ | 1818428239908962304 |
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| author | Ryota Sakamoto Vincent Noireaux Yusuke T. Maeda |
| author_facet | Ryota Sakamoto Vincent Noireaux Yusuke T. Maeda |
| author_sort | Ryota Sakamoto |
| collection | DOAJ |
| description | Abstract Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R 4 different from the expected size-dependence of R 3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression. |
| first_indexed | 2024-12-14T14:58:28Z |
| format | Article |
| id | doaj.art-3fed9043a938420d8689c93dd87d5455 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:58:28Z |
| publishDate | 2018-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3fed9043a938420d8689c93dd87d54552022-12-21T22:56:55ZengNature PortfolioScientific Reports2045-23222018-05-01811810.1038/s41598-018-25532-3Anomalous Scaling of Gene Expression in Confined Cell-Free ReactionsRyota Sakamoto0Vincent Noireaux1Yusuke T. Maeda2Department of Physics, Kyushu UniversitySchool of Physics and Astronomy, University of MinnesotaDepartment of Physics, Kyushu UniversityAbstract Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R 4 different from the expected size-dependence of R 3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.https://doi.org/10.1038/s41598-018-25532-3 |
| spellingShingle | Ryota Sakamoto Vincent Noireaux Yusuke T. Maeda Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions Scientific Reports |
| title | Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions |
| title_full | Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions |
| title_fullStr | Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions |
| title_full_unstemmed | Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions |
| title_short | Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions |
| title_sort | anomalous scaling of gene expression in confined cell free reactions |
| url | https://doi.org/10.1038/s41598-018-25532-3 |
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