Efficient identification of point-mutant monoclonal cells through array tagged high-throughput sequencing
Objective To establish an array tagged high-throughput sequencing and pre-polyclonal screening strategy for fast identification of genome-edited cell clones. Methods Introducing of G to T point mutation at rs826415 site of K562 cells by CRISPR/Cas9-mediated homologous recombination. The candidate...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-07-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-903.pdf |