CRISPR/Cas9-Mediated Specific Integration of <i>Fat-1</i> and <i>IGF-1</i> at the p<i>Rosa26</i> Locus

Many researchers have focused on knock-in pigs for site-specific integration, but little attention has been given to genetically modified pigs with the targeted integration of multiple recombinant genes. To establish a multigene targeted knock-in editing system, we used the internal ribosome entry s...

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Bibliographic Details
Main Authors: Wenni You, Mengjing Li, Yilin Qi, Yanbing Wang, Yiwu Chen, Ying Liu, Li Li, Hongsheng Ouyang, Daxin Pang
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Genes
Subjects:
Online Access:https://www.mdpi.com/2073-4425/12/7/1027