Efficient and cost-effective bacterial mRNA sequencing from low input samples through ribosomal RNA depletion

Efficient and cost-effective bacterial mRNA sequencing from low input samples through ribosomal RNA depletion

Abstract Background RNA sequencing is a powerful approach to quantify the genome-wide distribution of mRNA molecules in a population to gain deeper understanding of cellular functions and phenotypes. However, unlike eukaryotic cells, mRNA sequencing of bacterial samples is more challenging due to th...

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Bibliographic Details
Main Authors: Chatarin Wangsanuwat, Kellie A. Heom, Estella Liu, Michelle A. O’Malley, Siddharth S. Dey
Format: Article
Language:English
Published: BMC 2020-10-01
Series:BMC Genomics
Subjects:
Bacterial mRNA sequencing
mRNA enrichment
rRNA depletion
Low input total RNA
Online Access:http://link.springer.com/article/10.1186/s12864-020-07134-4

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http://link.springer.com/article/10.1186/s12864-020-07134-4

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