ESTABLISHMENT OF A RAPID SCREENING METHOD FOR IN VITRO SITE-DIRECTED MUTANTS OR CRISPR/Cas9-MEDIA-TED KNOCK-IN MUTANTS
Objective To establish a rapid screening method for in vitro site-directed mutants or CRISPR/Cas9-mediated knock-in mutants, and to improve mutant screening efficiency and reduce experimental costs. Methods Polymerase chain reactions (PCRs) were carried out with primers designed with the last one, t...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Precision Medicine
2023-06-01
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| Series: | 精准医学杂志 |
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| Online Access: | https://jpmed.qdu.edu.cn/fileup/2096-529X/PDF/202303015.pdf |