Two-Photon Excited Fluorescence of NADH-Alcohol Dehydrogenase Complex in a Mixture with Bacterial Enzymes

Two-Photon Excited Fluorescence of NADH-Alcohol Dehydrogenase Complex in a Mixture with Bacterial Enzymes

Thorough study of composition and fluorescence properties of a commercial reagent of active equine NAD-dependent alcohol dehydrogenase expressed and purified from <i>E. coli</i> has been carried out. Several experimental methods: spectral- and time-resolved two-photon excited fluorescenc...

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Bibliographic Details
Main Authors: Ioanna A. Gorbunova, Maxim E. Sasin, Dmitry V. Yachkov, Denis A. Volkov, Alexei D. Vedyaykin, Andrey A. Nikiforov, Oleg S. Vasyutinskii
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Biomolecules
Subjects:
NADH
PLP
dehydrogenases
fluorescence
TCSPC technique
intracellular biological fluorophores
Online Access:https://www.mdpi.com/2218-273X/13/2/256

Internet

https://www.mdpi.com/2218-273X/13/2/256

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