Improved Bacterial Single-Cell RNA-Seq through Automated MATQ-Seq and Cas9-Based Removal of rRNA Reads

Improved Bacterial Single-Cell RNA-Seq through Automated MATQ-Seq and Cas9-Based Removal of rRNA Reads

ABSTRACT Bulk RNA sequencing technologies have provided invaluable insights into host and bacterial gene expression and associated regulatory networks. Nevertheless, the majority of these approaches report average expression across cell populations, hiding the true underlying expression patterns tha...

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Bibliographic Details
Main Authors: Christina Homberger, Regan J. Hayward, Lars Barquist, Jörg Vogel
Format: Article
Language:English
Published: American Society for Microbiology 2023-04-01
Series:mBio
Subjects:
MATQ-seq
single-cell RNA-seq
Salmonella enterica
rRNA depletion
gene expression heterogeneity
DASH
Online Access:https://journals.asm.org/doi/10.1128/mbio.03557-22

Internet

https://journals.asm.org/doi/10.1128/mbio.03557-22

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