The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis
The major histocompatibility complex (MHC) is crucial for appropriate immune responses against invading pathogens. Chickens possess a single predominantly-expressed class I molecule with strong associations between disease resistance and MHC haplotype. For Marek’s disease virus (MDV) infections of c...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.908305/full |
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| author | Luca D. Bertzbach Clive A. Tregaskes Rebecca J. Martin Undine-Sophie Deumer Lan Huynh Ahmed M. Kheimar Ahmed M. Kheimar Andelé M. Conradie Jakob Trimpert Jim Kaufman Jim Kaufman Benedikt B. Kaufer Benedikt B. Kaufer |
| author_facet | Luca D. Bertzbach Clive A. Tregaskes Rebecca J. Martin Undine-Sophie Deumer Lan Huynh Ahmed M. Kheimar Ahmed M. Kheimar Andelé M. Conradie Jakob Trimpert Jim Kaufman Jim Kaufman Benedikt B. Kaufer Benedikt B. Kaufer |
| author_sort | Luca D. Bertzbach |
| collection | DOAJ |
| description | The major histocompatibility complex (MHC) is crucial for appropriate immune responses against invading pathogens. Chickens possess a single predominantly-expressed class I molecule with strong associations between disease resistance and MHC haplotype. For Marek’s disease virus (MDV) infections of chickens, the MHC haplotype is one of the major determinants of genetic resistance and susceptibility. VALO specific pathogen free (SPF) chickens are widely used in biomedical research and vaccine production. While valuable findings originate from MDV infections of VALO SPF chickens, their MHC haplotypes and associated disease resistance remained elusive. In this study, we used several typing systems to show that VALO SPF chickens possess MHC haplotypes that include B9, B9:02, B15, B19 and B21 at various frequencies. Moreover, we associate the MHC haplotypes to MDV-induced disease and lymphoma formation and found that B15 homozygotes had the lowest tumor incidence while B21 homozygotes had the lowest number of organs with tumors. Finally, we found transmission at variable levels to all contact birds except B15/B21 heterozygotes. These data have immediate implications for the use of VALO SPF chickens and eggs in the life sciences and add another piece to the puzzle of the chicken MHC complex and its role in infections with this oncogenic herpesvirus. |
| first_indexed | 2024-12-12T14:30:59Z |
| format | Article |
| id | doaj.art-4095716551bc420a801d0a73a8cb8715 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T14:30:59Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4095716551bc420a801d0a73a8cb87152022-12-22T00:21:32ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-05-011310.3389/fimmu.2022.908305908305The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and TumorigenesisLuca D. Bertzbach0Clive A. Tregaskes1Rebecca J. Martin2Undine-Sophie Deumer3Lan Huynh4Ahmed M. Kheimar5Ahmed M. Kheimar6Andelé M. Conradie7Jakob Trimpert8Jim Kaufman9Jim Kaufman10Benedikt B. Kaufer11Benedikt B. Kaufer12Institute of Virology, Freie Universität Berlin, Berlin, GermanyDepartment of Pathology, University of Cambridge, Cambridge, United KingdomDepartment of Pathology, University of Cambridge, Cambridge, United KingdomDepartment of Pathology, University of Cambridge, Cambridge, United KingdomInstitute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United KingdomInstitute of Virology, Freie Universität Berlin, Berlin, GermanyDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Sohag University, Sohag, EgyptInstitute of Virology, Freie Universität Berlin, Berlin, GermanyInstitute of Virology, Freie Universität Berlin, Berlin, GermanyDepartment of Pathology, University of Cambridge, Cambridge, United KingdomInstitute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United KingdomInstitute of Virology, Freie Universität Berlin, Berlin, GermanyVeterinary Centre for Resistance Research (TZR), Freie Universität Berlin, Berlin, GermanyThe major histocompatibility complex (MHC) is crucial for appropriate immune responses against invading pathogens. Chickens possess a single predominantly-expressed class I molecule with strong associations between disease resistance and MHC haplotype. For Marek’s disease virus (MDV) infections of chickens, the MHC haplotype is one of the major determinants of genetic resistance and susceptibility. VALO specific pathogen free (SPF) chickens are widely used in biomedical research and vaccine production. While valuable findings originate from MDV infections of VALO SPF chickens, their MHC haplotypes and associated disease resistance remained elusive. In this study, we used several typing systems to show that VALO SPF chickens possess MHC haplotypes that include B9, B9:02, B15, B19 and B21 at various frequencies. Moreover, we associate the MHC haplotypes to MDV-induced disease and lymphoma formation and found that B15 homozygotes had the lowest tumor incidence while B21 homozygotes had the lowest number of organs with tumors. Finally, we found transmission at variable levels to all contact birds except B15/B21 heterozygotes. These data have immediate implications for the use of VALO SPF chickens and eggs in the life sciences and add another piece to the puzzle of the chicken MHC complex and its role in infections with this oncogenic herpesvirus.https://www.frontiersin.org/articles/10.3389/fimmu.2022.908305/fullmajor histocompatibility complex (MHC)B9B15B19B21resistance |
| spellingShingle | Luca D. Bertzbach Clive A. Tregaskes Rebecca J. Martin Undine-Sophie Deumer Lan Huynh Ahmed M. Kheimar Ahmed M. Kheimar Andelé M. Conradie Jakob Trimpert Jim Kaufman Jim Kaufman Benedikt B. Kaufer Benedikt B. Kaufer The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis Frontiers in Immunology major histocompatibility complex (MHC) B9 B15 B19 B21 resistance |
| title | The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis |
| title_full | The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis |
| title_fullStr | The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis |
| title_full_unstemmed | The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis |
| title_short | The Diverse Major Histocompatibility Complex Haplotypes of a Common Commercial Chicken Line and Their Effect on Marek’s Disease Virus Pathogenesis and Tumorigenesis |
| title_sort | diverse major histocompatibility complex haplotypes of a common commercial chicken line and their effect on marek s disease virus pathogenesis and tumorigenesis |
| topic | major histocompatibility complex (MHC) B9 B15 B19 B21 resistance |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.908305/full |
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