Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector

Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector

Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field. Restriction-assembly is a combined method of restriction digestion and G...

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Bibliographic Details
Main Authors: Xiaojuan Guo, Yangyang Sun, Juan Chen, Xiaohui Zou, Wenzhe Hou, Wenjie Tan, Tao Hung, Zhuozhuang Lu
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Viruses
Subjects:
adenovirus
vector
construct
Gibson assembly
restriction enzyme
infectious clone
Online Access:https://www.mdpi.com/1999-4915/14/3/546

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https://www.mdpi.com/1999-4915/14/3/546

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