The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed
Abstract This study investigated the role of causative infectious agents in ulceration of the non-glandular part of the porcine stomach (pars oesophagea). In total, 150 stomachs from slaughter pigs were included, 75 from pigs that received a meal feed, 75 from pigs that received an equivalent pellet...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-024-01274-1 |
| _version_ | 1797273586272043008 |
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| author | Emily Taillieu Steff Taelman Sofie De Bruyckere Evy Goossens Ilias Chantziaras Christophe Van Steenkiste Peter Yde Steven Hanssens Dimitri De Meyer Wim Van Criekinge Michiel Stock Dominiek Maes Koen Chiers Freddy Haesebrouck |
| author_facet | Emily Taillieu Steff Taelman Sofie De Bruyckere Evy Goossens Ilias Chantziaras Christophe Van Steenkiste Peter Yde Steven Hanssens Dimitri De Meyer Wim Van Criekinge Michiel Stock Dominiek Maes Koen Chiers Freddy Haesebrouck |
| author_sort | Emily Taillieu |
| collection | DOAJ |
| description | Abstract This study investigated the role of causative infectious agents in ulceration of the non-glandular part of the porcine stomach (pars oesophagea). In total, 150 stomachs from slaughter pigs were included, 75 from pigs that received a meal feed, 75 from pigs that received an equivalent pelleted feed with a smaller particle size. The pars oesophagea was macroscopically examined after slaughter. (q)PCR assays for H. suis, F. gastrosuis and H. pylori-like organisms were performed, as well as 16S rRNA sequencing for pars oesophagea microbiome analyses. All 150 pig stomachs showed lesions. F. gastrosuis was detected in 115 cases (77%) and H. suis in 117 cases (78%), with 92 cases (61%) of co-infection; H. pylori-like organisms were detected in one case. Higher infectious loads of H. suis increased the odds of severe gastric lesions (OR = 1.14, p = 0.038), while the presence of H. suis infection in the pyloric gland zone increased the probability of pars oesophageal erosions [16.4% (95% CI 0.6–32.2%)]. The causal effect of H. suis was mediated by decreased pars oesophageal microbiome diversity [−1.9% (95% CI − 5.0–1.2%)], increased abundances of Veillonella and Campylobacter spp., and decreased abundances of Lactobacillus, Escherichia-Shigella, and Enterobacteriaceae spp. Higher infectious loads of F. gastrosuis in the pars oesophagea decreased the odds of severe gastric lesions (OR = 0.8, p = 0.0014). Feed pelleting had no significant impact on the prevalence of severe gastric lesions (OR = 1.72, p = 0.28). H. suis infections are a risk factor for ulceration of the porcine pars oesophagea, probably mediated through alterations in pars oesophageal microbiome diversity and composition. |
| first_indexed | 2024-03-07T14:46:31Z |
| format | Article |
| id | doaj.art-40e5c094647a463f96d14a93fc01411f |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-07T14:46:31Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-40e5c094647a463f96d14a93fc01411f2024-03-05T19:58:27ZengBMCVeterinary Research1297-97162024-02-0155111610.1186/s13567-024-01274-1The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feedEmily Taillieu0Steff Taelman1Sofie De Bruyckere2Evy Goossens3Ilias Chantziaras4Christophe Van Steenkiste5Peter Yde6Steven Hanssens7Dimitri De Meyer8Wim Van Criekinge9Michiel Stock10Dominiek Maes11Koen Chiers12Freddy Haesebrouck13Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Data Analysis and Mathematical Modelling, BIOBIX, Ghent UniversityDepartment of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Gastroenterology and Hepatology, University Hospital Antwerp, Antwerp UniversityDanis NvDanis NvVedanko BvbaDepartment of Data Analysis and Mathematical Modelling, BIOBIX, Ghent UniversityDepartment of Data Analysis and Mathematical Modelling, BIOBIX, Ghent UniversityDepartment of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent UniversityAbstract This study investigated the role of causative infectious agents in ulceration of the non-glandular part of the porcine stomach (pars oesophagea). In total, 150 stomachs from slaughter pigs were included, 75 from pigs that received a meal feed, 75 from pigs that received an equivalent pelleted feed with a smaller particle size. The pars oesophagea was macroscopically examined after slaughter. (q)PCR assays for H. suis, F. gastrosuis and H. pylori-like organisms were performed, as well as 16S rRNA sequencing for pars oesophagea microbiome analyses. All 150 pig stomachs showed lesions. F. gastrosuis was detected in 115 cases (77%) and H. suis in 117 cases (78%), with 92 cases (61%) of co-infection; H. pylori-like organisms were detected in one case. Higher infectious loads of H. suis increased the odds of severe gastric lesions (OR = 1.14, p = 0.038), while the presence of H. suis infection in the pyloric gland zone increased the probability of pars oesophageal erosions [16.4% (95% CI 0.6–32.2%)]. The causal effect of H. suis was mediated by decreased pars oesophageal microbiome diversity [−1.9% (95% CI − 5.0–1.2%)], increased abundances of Veillonella and Campylobacter spp., and decreased abundances of Lactobacillus, Escherichia-Shigella, and Enterobacteriaceae spp. Higher infectious loads of F. gastrosuis in the pars oesophagea decreased the odds of severe gastric lesions (OR = 0.8, p = 0.0014). Feed pelleting had no significant impact on the prevalence of severe gastric lesions (OR = 1.72, p = 0.28). H. suis infections are a risk factor for ulceration of the porcine pars oesophagea, probably mediated through alterations in pars oesophageal microbiome diversity and composition.https://doi.org/10.1186/s13567-024-01274-1Sus scrofa domesticusstomachgastric ulcerationmicrobiotaHelicobacter suisFusobacterium gastrosuis |
| spellingShingle | Emily Taillieu Steff Taelman Sofie De Bruyckere Evy Goossens Ilias Chantziaras Christophe Van Steenkiste Peter Yde Steven Hanssens Dimitri De Meyer Wim Van Criekinge Michiel Stock Dominiek Maes Koen Chiers Freddy Haesebrouck The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed Veterinary Research Sus scrofa domesticus stomach gastric ulceration microbiota Helicobacter suis Fusobacterium gastrosuis |
| title | The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed |
| title_full | The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed |
| title_fullStr | The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed |
| title_full_unstemmed | The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed |
| title_short | The role of Helicobacter suis, Fusobacterium gastrosuis, and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed |
| title_sort | role of helicobacter suis fusobacterium gastrosuis and the pars oesophageal microbiota in gastric ulceration in slaughter pigs receiving meal or pelleted feed |
| topic | Sus scrofa domesticus stomach gastric ulceration microbiota Helicobacter suis Fusobacterium gastrosuis |
| url | https://doi.org/10.1186/s13567-024-01274-1 |
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