Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts
Abstract Purpose We aimed to study the association between adjusted mtDNA levels in human trophectoderm biopsy samples and the developmental potential of euploid and mosaic blastocysts. Methods We analyzed relative mtDNA levels in 2,814 blastocysts obtained from 576 couples undergoing preimplantatio...
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BMC
2023-06-01
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| Series: | BMC Pregnancy and Childbirth |
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| Online Access: | https://doi.org/10.1186/s12884-023-05760-w |
| _version_ | 1797801166612987904 |
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| author | Wen Luo Yi-Min Zheng Yan Hao Ying Zhang Ping Zhou Zhaolian Wei Yunxia Cao Dawei Chen |
| author_facet | Wen Luo Yi-Min Zheng Yan Hao Ying Zhang Ping Zhou Zhaolian Wei Yunxia Cao Dawei Chen |
| author_sort | Wen Luo |
| collection | DOAJ |
| description | Abstract Purpose We aimed to study the association between adjusted mtDNA levels in human trophectoderm biopsy samples and the developmental potential of euploid and mosaic blastocysts. Methods We analyzed relative mtDNA levels in 2,814 blastocysts obtained from 576 couples undergoing preimplantation genetic testing for aneuploidy from June 2018 to June 2021. All patients underwent in vitro fertilization in a single clinic; the study was blinded—mtDNA content was unknown at the time of single embryo transfer. The fate of the euploid or mosaic embryos transferred was compared with mtDNA levels. Results Euploid embryos had lower mtDNA than aneuploid and mosaic embryos. Embryos biopsied on Day 5 had higher mtDNA than those biopsied on Day 6. No difference was detected in mtDNA scores between embryos derived from oocytes of different maternal ages. Linear mixed model suggested that blastulation rate was associated with mtDNA score. Moreover, the specific next-generation sequencing platform used have a significant effect on the observed mtDNA content. Euploid embryos with higher mtDNA content presented significantly higher miscarriage rates and lower live birth rates, while no significant difference was observed in the mosaic cohort. Conclusion Our results will aid in improving methods for analyzing the association between mtDNA level and blastocyst viability. |
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| id | doaj.art-41142f413d164305b6b0e64d58b64e02 |
| institution | Directory Open Access Journal |
| issn | 1471-2393 |
| language | English |
| last_indexed | 2024-03-13T04:46:18Z |
| publishDate | 2023-06-01 |
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| series | BMC Pregnancy and Childbirth |
| spelling | doaj.art-41142f413d164305b6b0e64d58b64e022023-06-18T11:27:39ZengBMCBMC Pregnancy and Childbirth1471-23932023-06-0123111610.1186/s12884-023-05760-wMitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocystsWen Luo0Yi-Min Zheng1Yan Hao2Ying Zhang3Ping Zhou4Zhaolian Wei5Yunxia Cao6Dawei Chen7Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityAbstract Purpose We aimed to study the association between adjusted mtDNA levels in human trophectoderm biopsy samples and the developmental potential of euploid and mosaic blastocysts. Methods We analyzed relative mtDNA levels in 2,814 blastocysts obtained from 576 couples undergoing preimplantation genetic testing for aneuploidy from June 2018 to June 2021. All patients underwent in vitro fertilization in a single clinic; the study was blinded—mtDNA content was unknown at the time of single embryo transfer. The fate of the euploid or mosaic embryos transferred was compared with mtDNA levels. Results Euploid embryos had lower mtDNA than aneuploid and mosaic embryos. Embryos biopsied on Day 5 had higher mtDNA than those biopsied on Day 6. No difference was detected in mtDNA scores between embryos derived from oocytes of different maternal ages. Linear mixed model suggested that blastulation rate was associated with mtDNA score. Moreover, the specific next-generation sequencing platform used have a significant effect on the observed mtDNA content. Euploid embryos with higher mtDNA content presented significantly higher miscarriage rates and lower live birth rates, while no significant difference was observed in the mosaic cohort. Conclusion Our results will aid in improving methods for analyzing the association between mtDNA level and blastocyst viability.https://doi.org/10.1186/s12884-023-05760-wMitochondrionMitochondrial genomemtDNA quantificationBlastocystEmbryo viabilityPreimplantation genetic testing |
| spellingShingle | Wen Luo Yi-Min Zheng Yan Hao Ying Zhang Ping Zhou Zhaolian Wei Yunxia Cao Dawei Chen Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts BMC Pregnancy and Childbirth Mitochondrion Mitochondrial genome mtDNA quantification Blastocyst Embryo viability Preimplantation genetic testing |
| title | Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts |
| title_full | Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts |
| title_fullStr | Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts |
| title_full_unstemmed | Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts |
| title_short | Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts |
| title_sort | mitochondrial dna quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts |
| topic | Mitochondrion Mitochondrial genome mtDNA quantification Blastocyst Embryo viability Preimplantation genetic testing |
| url | https://doi.org/10.1186/s12884-023-05760-w |
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