Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation
Abstract The plant‐based medicine and diet is gaining importance in recent days. The consumption of Triticum aestivum grass in the form of juice and tablets is increasing among common people. The present study elaborates on the nutritional, antioxidant, and antimicrobial potential of a nongeneticall...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.233 |
| _version_ | 1797808942005354496 |
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| author | Mohandass Kaviya Balasubramanian Balamuralikrishnan Thangavelu Sangeetha Natchiappan Senthilkumar Arunkumar Malaisamy Murugasamy Sivasamy Loganathan Poorni Karthika Pushparaj Meyyazhagan Arun Arumugam Vijaya Anand |
| author_facet | Mohandass Kaviya Balasubramanian Balamuralikrishnan Thangavelu Sangeetha Natchiappan Senthilkumar Arunkumar Malaisamy Murugasamy Sivasamy Loganathan Poorni Karthika Pushparaj Meyyazhagan Arun Arumugam Vijaya Anand |
| author_sort | Mohandass Kaviya |
| collection | DOAJ |
| description | Abstract The plant‐based medicine and diet is gaining importance in recent days. The consumption of Triticum aestivum grass in the form of juice and tablets is increasing among common people. The present study elaborates on the nutritional, antioxidant, and antimicrobial potential of a nongenetically modified type of T. aestivum grass, along with the evidence of molecular docking studies. The T. aestivum grass extracts like decoction, aqueous, ethanol, and chloroform were subjected to preliminary phytochemical tests, quantitative estimation, antioxidant analysis, and antimicrobial activity determination. The ethanolic extract that had good antioxidant and antimicrobial potential was subjected to gas column–mass spectroscopy (GC–MS) analysis and the compounds identified were docked against the antioxidant and antimicrobial receptors. The decoction and aqueous extracts performed well in preliminary qualitative tests with the presence of most of the phytochemicals tested. The decoction, aqueous, and ethanolic extracts possessed good concentrations of the phytochemicals. The decoction had about 210.83 ± 9.16 and 154.16 ± 0.33 mg/g of carbohydrates and proteins, respectively, while the aqueous extract had about 10.91 ± 0.08 mg/g of amino acids and the ethanolic extract had about 52.5 ± 1.4 mg/g of phenolic content, which were the highest concentration of the phytochemicals observed among the extracts. Along with phytochemical potential, good antioxidant potential in the DPPH and ABTS by decoction as well as ethanolic extract with nearly 40 and 90% inhibition, respectively, and in FRAP by aqueous extract with maximum OD value. The ethanolic extract exhibited the best inhibition potential against the Staphylococcus aureus about 28 ± 1 mm, Pseudomonas aeruginosa with 20 ± 2 mm, Bacillus cereus at 20 ± 1 mm by the ethanolic extract at 200 μg concentration, and Aspergillus fumigatus and A. niger at 15 ± 0 mm by the aqueous extract at 200 μg concentration. The GC–MS analysis revealed the presence of terpenoids, alkaloids, and phenols, which on docking had highest binding capacity toward the antioxidant and antimicrobial receptors. |
| first_indexed | 2024-03-13T06:45:44Z |
| format | Article |
| id | doaj.art-41412e7f5c694d3ba513d1b8d8d749da |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-03-13T06:45:44Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-41412e7f5c694d3ba513d1b8d8d749da2023-06-08T06:14:55ZengWileyFood Frontiers2643-84292023-06-014283184810.1002/fft2.233Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigationMohandass Kaviya0Balasubramanian Balamuralikrishnan1Thangavelu Sangeetha2Natchiappan Senthilkumar3Arunkumar Malaisamy4Murugasamy Sivasamy5Loganathan Poorni6Karthika Pushparaj7Meyyazhagan Arun8Arumugam Vijaya Anand9Department of Human Genetics and Molecular Biology Bharathiar University Coimbatore Tamil Nadu IndiaDepartment of Food Science and Biotechnology College of Life Science Sejong University Seoul South KoreaDepartment of Human Genetics and Molecular Biology Bharathiar University Coimbatore Tamil Nadu IndiaDepartment of Bioprospecting of Forest Resources, Institute of Forest Genetics and Tree Breeding (IFGTB) Forest Campus Coimbatore Tamil Nadu IndiaTranscription Regulation Group International Centre for Genetic Engineering and Biotechnology (ICGEB) New Delhi IndiaDivision of Crops Improvement Indian Council of Agricultural Research (ICAR) ‐ Indian Agricultural Research Institute, Regional Station, Wellington Nilgiris Tamil Nadu IndiaDepartment of Biochemistry Vivekanandha College of Arts and Sciences for Women Namakkal Tamil Nadu IndiaDepartment of Zoology School of Biosciences Avinashilingam Institute for Home Science and Higher Education for Women Coimbatore Tamil Nadu IndiaDepartment of Life Sciences CHRIST (Deemed to be University) Bengaluru Karnataka IndiaDepartment of Human Genetics and Molecular Biology Bharathiar University Coimbatore Tamil Nadu IndiaAbstract The plant‐based medicine and diet is gaining importance in recent days. The consumption of Triticum aestivum grass in the form of juice and tablets is increasing among common people. The present study elaborates on the nutritional, antioxidant, and antimicrobial potential of a nongenetically modified type of T. aestivum grass, along with the evidence of molecular docking studies. The T. aestivum grass extracts like decoction, aqueous, ethanol, and chloroform were subjected to preliminary phytochemical tests, quantitative estimation, antioxidant analysis, and antimicrobial activity determination. The ethanolic extract that had good antioxidant and antimicrobial potential was subjected to gas column–mass spectroscopy (GC–MS) analysis and the compounds identified were docked against the antioxidant and antimicrobial receptors. The decoction and aqueous extracts performed well in preliminary qualitative tests with the presence of most of the phytochemicals tested. The decoction, aqueous, and ethanolic extracts possessed good concentrations of the phytochemicals. The decoction had about 210.83 ± 9.16 and 154.16 ± 0.33 mg/g of carbohydrates and proteins, respectively, while the aqueous extract had about 10.91 ± 0.08 mg/g of amino acids and the ethanolic extract had about 52.5 ± 1.4 mg/g of phenolic content, which were the highest concentration of the phytochemicals observed among the extracts. Along with phytochemical potential, good antioxidant potential in the DPPH and ABTS by decoction as well as ethanolic extract with nearly 40 and 90% inhibition, respectively, and in FRAP by aqueous extract with maximum OD value. The ethanolic extract exhibited the best inhibition potential against the Staphylococcus aureus about 28 ± 1 mm, Pseudomonas aeruginosa with 20 ± 2 mm, Bacillus cereus at 20 ± 1 mm by the ethanolic extract at 200 μg concentration, and Aspergillus fumigatus and A. niger at 15 ± 0 mm by the aqueous extract at 200 μg concentration. The GC–MS analysis revealed the presence of terpenoids, alkaloids, and phenols, which on docking had highest binding capacity toward the antioxidant and antimicrobial receptors.https://doi.org/10.1002/fft2.233carbohydratesdiphenyl‐1‐picryl‐hydrazylgas column–mass spectroscopyproteinsPseudomonas aeruginosaStaphylococcus aureus |
| spellingShingle | Mohandass Kaviya Balasubramanian Balamuralikrishnan Thangavelu Sangeetha Natchiappan Senthilkumar Arunkumar Malaisamy Murugasamy Sivasamy Loganathan Poorni Karthika Pushparaj Meyyazhagan Arun Arumugam Vijaya Anand Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation Food Frontiers carbohydrates diphenyl‐1‐picryl‐hydrazyl gas column–mass spectroscopy proteins Pseudomonas aeruginosa Staphylococcus aureus |
| title | Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation |
| title_full | Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation |
| title_fullStr | Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation |
| title_full_unstemmed | Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation |
| title_short | Evaluation of phytoconstituents of Triticum aestivum grass extracts on nutritional attributes, antioxidant, and antimicrobial activities against food pathogens with molecular in silico investigation |
| title_sort | evaluation of phytoconstituents of triticum aestivum grass extracts on nutritional attributes antioxidant and antimicrobial activities against food pathogens with molecular in silico investigation |
| topic | carbohydrates diphenyl‐1‐picryl‐hydrazyl gas column–mass spectroscopy proteins Pseudomonas aeruginosa Staphylococcus aureus |
| url | https://doi.org/10.1002/fft2.233 |
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