Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency
Mouse epiblast stem cells (mEpiSCs) and human embryonic stem cells (hESCs) are primed pluripotent stem cells whose self-renewal can be maintained through cytoplasmic stabilization and retention of β-catenin. The underlying mechanism, however, remains largely unknown. Here, we show that cytoplasmic β...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117303296 |
| _version_ | 1828921513343975424 |
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| author | Xingliang Zhou Jean Paul Chadarevian Bryan Ruiz Qi-Long Ying |
| author_facet | Xingliang Zhou Jean Paul Chadarevian Bryan Ruiz Qi-Long Ying |
| author_sort | Xingliang Zhou |
| collection | DOAJ |
| description | Mouse epiblast stem cells (mEpiSCs) and human embryonic stem cells (hESCs) are primed pluripotent stem cells whose self-renewal can be maintained through cytoplasmic stabilization and retention of β-catenin. The underlying mechanism, however, remains largely unknown. Here, we show that cytoplasmic β-catenin interacts with and retains TAZ, a Hippo pathway effector, in the cytoplasm. Cytoplasmic retention of TAZ promotes mEpiSC self-renewal in the absence of nuclear β-catenin, whereas nuclear translocation of TAZ induces mEpiSC differentiation. TAZ is dispensable for naive mouse embryonic stem cell (mESC) self-renewal but required for the proper conversion of mESCs to mEpiSCs. The self-renewal of hESCs, like that of mEpiSCs, can also be maintained through the cytoplasmic retention of β-catenin and TAZ. Our study indicates that how TAZ regulates cell fate depends on not only the cell type but also its subcellular localization. |
| first_indexed | 2024-12-13T22:03:56Z |
| format | Article |
| id | doaj.art-414631d1e9544a35a7e51de1ed0618b0 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-13T22:03:56Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-414631d1e9544a35a7e51de1ed0618b02022-12-21T23:29:54ZengElsevierStem Cell Reports2213-67112017-09-019373274110.1016/j.stemcr.2017.07.019Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed PluripotencyXingliang Zhou0Jean Paul Chadarevian1Bryan Ruiz2Qi-Long Ying3Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USAEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USAEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USAEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USAMouse epiblast stem cells (mEpiSCs) and human embryonic stem cells (hESCs) are primed pluripotent stem cells whose self-renewal can be maintained through cytoplasmic stabilization and retention of β-catenin. The underlying mechanism, however, remains largely unknown. Here, we show that cytoplasmic β-catenin interacts with and retains TAZ, a Hippo pathway effector, in the cytoplasm. Cytoplasmic retention of TAZ promotes mEpiSC self-renewal in the absence of nuclear β-catenin, whereas nuclear translocation of TAZ induces mEpiSC differentiation. TAZ is dispensable for naive mouse embryonic stem cell (mESC) self-renewal but required for the proper conversion of mESCs to mEpiSCs. The self-renewal of hESCs, like that of mEpiSCs, can also be maintained through the cytoplasmic retention of β-catenin and TAZ. Our study indicates that how TAZ regulates cell fate depends on not only the cell type but also its subcellular localization.http://www.sciencedirect.com/science/article/pii/S2213671117303296β-cateninTAZHippo pathwayWnt signaling pathwayepiblast stem cellhuman embryonic stem cellprimed pluripotencystem cell self-renewal |
| spellingShingle | Xingliang Zhou Jean Paul Chadarevian Bryan Ruiz Qi-Long Ying Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency Stem Cell Reports β-catenin TAZ Hippo pathway Wnt signaling pathway epiblast stem cell human embryonic stem cell primed pluripotency stem cell self-renewal |
| title | Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency |
| title_full | Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency |
| title_fullStr | Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency |
| title_full_unstemmed | Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency |
| title_short | Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency |
| title_sort | cytoplasmic and nuclear taz exert distinct functions in regulating primed pluripotency |
| topic | β-catenin TAZ Hippo pathway Wnt signaling pathway epiblast stem cell human embryonic stem cell primed pluripotency stem cell self-renewal |
| url | http://www.sciencedirect.com/science/article/pii/S2213671117303296 |
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