Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan
Taxus wallichiana (Himalayan yew) antioxidant potential enhances the release of secondary metabolites and enzymes under stress; over the last few decades owing to changes in climatic regimes, such species are under constant threat in the moist temperate Himalayan forests. The present study aims to e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2024-02-01
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| Series: | Journal of Water and Climate Change |
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| Online Access: | http://jwcc.iwaponline.com/content/15/2/669 |
| _version_ | 1797202735448195072 |
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| author | Sanam Zarif Satti Samina Siddiqui Asim Shahzad Wadood Shah |
| author_facet | Sanam Zarif Satti Samina Siddiqui Asim Shahzad Wadood Shah |
| author_sort | Sanam Zarif Satti |
| collection | DOAJ |
| description | Taxus wallichiana (Himalayan yew) antioxidant potential enhances the release of secondary metabolites and enzymes under stress; over the last few decades owing to changes in climatic regimes, such species are under constant threat in the moist temperate Himalayan forests. The present study aims to evaluate the effect of change in land-use pattern on the antioxidant and phytochemical potential of T. wallichiana (Himalayan yew) in the moist temperate Himalayan Forest of Galiyat-Khyber-Pakhtunkhwa-Pakistan. Three leaf samples from each location of T.W were collected from high (Ayubiya, 2,970 m.a.s.l.) undisturbed, disturbed mid (Baragali, Dongagali, Kuldana, Chegagali, 2,617, 2,375, 2,455, 2,804 m.a.s.l.) and low (Murree, 2,000 m.a.s.l.) altitudes of moist temperate forest of Galiayt-Himalayan-Khyber-Pakhtunkhwa-Pakistan, DPPH assay, total flavonoids and phenolic content, total protein and proline content, catalase, superoxide dismutase and peroxidase activities were analysed. The antioxidant activity (DPPH) response was more pronounced in low and mid altitude disturbed sites than the undisturbed site at high altitudes. Antioxidant enzymes and osmolyte content further supported the stress tolerance capacity of T. wallichiana to scavenge the ROS produced under oxidative stress conditions. In conclusion, Taxus wallichiana inhabiting in these sites could withstand long durations of drought, salinity, frost, high temperatures, and pathogenic attacks by activating the antioxidant enzymes.
HIGHLIGHTS
The biggest threat to the Taxus wallichiana population is overexploitation, deforestation and changing climatic regimes leading to droughts.;
Taxus wallichiana leaf samples collected from Ayubia and Murree regions showed maximum levels of antioxidant enzymes and osmolyte content thus validating the scientific fact that Taxus wallichiana growing in these regions are stress-tolerant and can withstand long duration of oxidative stress.; |
| first_indexed | 2024-04-24T08:08:10Z |
| format | Article |
| id | doaj.art-414cd10bb6e24c67976108d29a9c0310 |
| institution | Directory Open Access Journal |
| issn | 2040-2244 2408-9354 |
| language | English |
| last_indexed | 2024-04-24T08:08:10Z |
| publishDate | 2024-02-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Journal of Water and Climate Change |
| spelling | doaj.art-414cd10bb6e24c67976108d29a9c03102024-04-17T08:46:33ZengIWA PublishingJournal of Water and Climate Change2040-22442408-93542024-02-0115266968510.2166/wcc.2023.539539Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, PakistanSanam Zarif Satti0Samina Siddiqui1Asim Shahzad2Wadood Shah3 National Centre of Excellence in Geology, University of Peshawar, Peshwar 25120, Pakistan National Centre of Excellence in Geology, University of Peshawar, Peshwar 25120, Pakistan The College of Geography and Environmental Science, Henan University, Jinming Ave, Kaifeng 475004, China Biological Sciences Research Division, Pakistan Forest Institute, Peshawar 25120, Pakistan Taxus wallichiana (Himalayan yew) antioxidant potential enhances the release of secondary metabolites and enzymes under stress; over the last few decades owing to changes in climatic regimes, such species are under constant threat in the moist temperate Himalayan forests. The present study aims to evaluate the effect of change in land-use pattern on the antioxidant and phytochemical potential of T. wallichiana (Himalayan yew) in the moist temperate Himalayan Forest of Galiyat-Khyber-Pakhtunkhwa-Pakistan. Three leaf samples from each location of T.W were collected from high (Ayubiya, 2,970 m.a.s.l.) undisturbed, disturbed mid (Baragali, Dongagali, Kuldana, Chegagali, 2,617, 2,375, 2,455, 2,804 m.a.s.l.) and low (Murree, 2,000 m.a.s.l.) altitudes of moist temperate forest of Galiayt-Himalayan-Khyber-Pakhtunkhwa-Pakistan, DPPH assay, total flavonoids and phenolic content, total protein and proline content, catalase, superoxide dismutase and peroxidase activities were analysed. The antioxidant activity (DPPH) response was more pronounced in low and mid altitude disturbed sites than the undisturbed site at high altitudes. Antioxidant enzymes and osmolyte content further supported the stress tolerance capacity of T. wallichiana to scavenge the ROS produced under oxidative stress conditions. In conclusion, Taxus wallichiana inhabiting in these sites could withstand long durations of drought, salinity, frost, high temperatures, and pathogenic attacks by activating the antioxidant enzymes. HIGHLIGHTS The biggest threat to the Taxus wallichiana population is overexploitation, deforestation and changing climatic regimes leading to droughts.; Taxus wallichiana leaf samples collected from Ayubia and Murree regions showed maximum levels of antioxidant enzymes and osmolyte content thus validating the scientific fact that Taxus wallichiana growing in these regions are stress-tolerant and can withstand long duration of oxidative stress.;http://jwcc.iwaponline.com/content/15/2/669antioxidant enzymesphysio-biochemicalreactive oxygen speciestaxus wallichiana (himalayan yew) |
| spellingShingle | Sanam Zarif Satti Samina Siddiqui Asim Shahzad Wadood Shah Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan Journal of Water and Climate Change antioxidant enzymes physio-biochemical reactive oxygen species taxus wallichiana (himalayan yew) |
| title | Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan |
| title_full | Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan |
| title_fullStr | Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan |
| title_full_unstemmed | Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan |
| title_short | Accelerated anti-oxidant enzymes and phytochemical potential of Taxus wallichiana (Himalayan yew) under moist temperate forest of Himalayan, Pakistan |
| title_sort | accelerated anti oxidant enzymes and phytochemical potential of taxus wallichiana himalayan yew under moist temperate forest of himalayan pakistan |
| topic | antioxidant enzymes physio-biochemical reactive oxygen species taxus wallichiana (himalayan yew) |
| url | http://jwcc.iwaponline.com/content/15/2/669 |
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