Behavioral characterization in MPTP/p mouse model of Parkinson’s disease
We evaluated the practicability of using the rarely utilized C57BL/6N mouse as a Parkinson’s disease model established via the acute MPTP/probenecid (MPTP/p) protocol. We confirmed dopaminergic degeneration in terms of decreased expression levels of tyrosine hydroxylase in the substantia nigra a...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2021-06-01
|
| Series: | Journal of Integrative Neuroscience |
| Subjects: | |
| Online Access: | https://jin.imrpress.com/fileup/1757-448X/PDF/1625014728953-1784839714.pdf |
| _version_ | 1828748502096674816 |
|---|---|
| author | Mai Wada Mary Jasmin Ang Poornima D. E. Weerasinghe-Mudiyanselage Sung-Ho Kim Jong-Choon Kim Taekyun Shin Changjong Moon |
| author_facet | Mai Wada Mary Jasmin Ang Poornima D. E. Weerasinghe-Mudiyanselage Sung-Ho Kim Jong-Choon Kim Taekyun Shin Changjong Moon |
| author_sort | Mai Wada |
| collection | DOAJ |
| description | We evaluated the practicability of using the rarely utilized C57BL/6N
mouse as a Parkinson’s disease model established via the acute MPTP/probenecid
(MPTP/p) protocol. We confirmed dopaminergic degeneration in terms of decreased
expression levels of tyrosine hydroxylase in the substantia nigra and striatum of
MPTP/p-lesioned mice. In addition, acute MPTP/p-lesioned mice demonstrated
initial motor dysfunctions followed by spontaneous recovery. Interestingly, these
MPTP/p-lesioned mice exhibited anxiolytic and antidepressive behaviors upon
recovery from these motor deficits. Additionally, increased expression of
norepinephrine transporters in several brain regions, including the hippocampus,
medial prefrontal cortex, and striatum, and an elevated rate of adult
neurogenesis (in terms of increased numbers of doublecortin-positive neuroblasts)
in the hippocampus were observed after recovery from motor dysfunctions. We
suggest that the emotional alterations observed under these experimental
conditions may be associated with enhanced adult neurogenesis, increased levels
of norepinephrine transporters, and/or a possible interplay between these two
factors. Consequently, this acute MPTP/p model adequately satisfies the criteria
for the validity of a Parkinson’s disease model regarding dopaminergic loss and
motor impairment. However, the non-motor findings may offer novel evidence
against the practicability of utilizing the acute MPTP/p-lesioned mice for
modeling the emotional aberrations found in Parkinson’s disease patients. |
| first_indexed | 2024-04-14T05:02:28Z |
| format | Article |
| id | doaj.art-41534c932f1b455dbbb339358909b244 |
| institution | Directory Open Access Journal |
| issn | 1757-448X |
| language | English |
| last_indexed | 2024-04-14T05:02:28Z |
| publishDate | 2021-06-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-41534c932f1b455dbbb339358909b2442022-12-22T02:10:52ZengIMR PressJournal of Integrative Neuroscience1757-448X2021-06-0120230732010.31083/j.jin20020301625014728953-1784839714Behavioral characterization in MPTP/p mouse model of Parkinson’s diseaseMai Wada0Mary Jasmin Ang1Poornima D. E. Weerasinghe-Mudiyanselage2Sung-Ho Kim3Jong-Choon Kim4Taekyun Shin5Changjong Moon6Department of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, 61186 Gwangju, South KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, 61186 Gwangju, South KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, 61186 Gwangju, South KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, 61186 Gwangju, South KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, 61186 Gwangju, South KoreaDepartment of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, 63243 Jeju, South KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, 61186 Gwangju, South KoreaWe evaluated the practicability of using the rarely utilized C57BL/6N mouse as a Parkinson’s disease model established via the acute MPTP/probenecid (MPTP/p) protocol. We confirmed dopaminergic degeneration in terms of decreased expression levels of tyrosine hydroxylase in the substantia nigra and striatum of MPTP/p-lesioned mice. In addition, acute MPTP/p-lesioned mice demonstrated initial motor dysfunctions followed by spontaneous recovery. Interestingly, these MPTP/p-lesioned mice exhibited anxiolytic and antidepressive behaviors upon recovery from these motor deficits. Additionally, increased expression of norepinephrine transporters in several brain regions, including the hippocampus, medial prefrontal cortex, and striatum, and an elevated rate of adult neurogenesis (in terms of increased numbers of doublecortin-positive neuroblasts) in the hippocampus were observed after recovery from motor dysfunctions. We suggest that the emotional alterations observed under these experimental conditions may be associated with enhanced adult neurogenesis, increased levels of norepinephrine transporters, and/or a possible interplay between these two factors. Consequently, this acute MPTP/p model adequately satisfies the criteria for the validity of a Parkinson’s disease model regarding dopaminergic loss and motor impairment. However, the non-motor findings may offer novel evidence against the practicability of utilizing the acute MPTP/p-lesioned mice for modeling the emotional aberrations found in Parkinson’s disease patients.https://jin.imrpress.com/fileup/1757-448X/PDF/1625014728953-1784839714.pdfbrain regionsmotor symptomneurogenesisnorepinephrine transporternon-motor symptomparkinson's disease |
| spellingShingle | Mai Wada Mary Jasmin Ang Poornima D. E. Weerasinghe-Mudiyanselage Sung-Ho Kim Jong-Choon Kim Taekyun Shin Changjong Moon Behavioral characterization in MPTP/p mouse model of Parkinson’s disease Journal of Integrative Neuroscience brain regions motor symptom neurogenesis norepinephrine transporter non-motor symptom parkinson's disease |
| title | Behavioral characterization in MPTP/p mouse model of Parkinson’s disease |
| title_full | Behavioral characterization in MPTP/p mouse model of Parkinson’s disease |
| title_fullStr | Behavioral characterization in MPTP/p mouse model of Parkinson’s disease |
| title_full_unstemmed | Behavioral characterization in MPTP/p mouse model of Parkinson’s disease |
| title_short | Behavioral characterization in MPTP/p mouse model of Parkinson’s disease |
| title_sort | behavioral characterization in mptp p mouse model of parkinson s disease |
| topic | brain regions motor symptom neurogenesis norepinephrine transporter non-motor symptom parkinson's disease |
| url | https://jin.imrpress.com/fileup/1757-448X/PDF/1625014728953-1784839714.pdf |
| work_keys_str_mv | AT maiwada behavioralcharacterizationinmptppmousemodelofparkinsonsdisease AT maryjasminang behavioralcharacterizationinmptppmousemodelofparkinsonsdisease AT poornimadeweerasinghemudiyanselage behavioralcharacterizationinmptppmousemodelofparkinsonsdisease AT sunghokim behavioralcharacterizationinmptppmousemodelofparkinsonsdisease AT jongchoonkim behavioralcharacterizationinmptppmousemodelofparkinsonsdisease AT taekyunshin behavioralcharacterizationinmptppmousemodelofparkinsonsdisease AT changjongmoon behavioralcharacterizationinmptppmousemodelofparkinsonsdisease |