Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia
Oxidative stress caused by chemicals brings about mass mortalities in aquatic organisms and in this context, antioxidants and heat shock protein 70 (Hsp70) stimulating compounds represent potential alternatives to alleviate this problem during aquaculture. The antioxidant capacity and efficacies of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513420305238 |
| _version_ | 1811323083906088960 |
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| author | Irene Kai Ru Tiong Thilahgavani Nagappan Mohd Effendy Abdul Wahid Tengku Sifzizul Tengku Muhammad Toda Tatsuki Woro Hastuti Satyantini Gunanti Mahasri Patrick Sorgeloos Yeong Yik Sung |
| author_facet | Irene Kai Ru Tiong Thilahgavani Nagappan Mohd Effendy Abdul Wahid Tengku Sifzizul Tengku Muhammad Toda Tatsuki Woro Hastuti Satyantini Gunanti Mahasri Patrick Sorgeloos Yeong Yik Sung |
| author_sort | Irene Kai Ru Tiong |
| collection | DOAJ |
| description | Oxidative stress caused by chemicals brings about mass mortalities in aquatic organisms and in this context, antioxidants and heat shock protein 70 (Hsp70) stimulating compounds represent potential alternatives to alleviate this problem during aquaculture. The antioxidant capacity and efficacies of five Malaysian indigenous microalgae, namely Auxenochlorella pyrenoidosa, Chlorella vulgaris, Messastrum gracile, Desmodesmus subspicatus and Parachlorella kessleri to regulate Hsp70 expression in the brine shrimp Artemia, an important live food species, were examined. A. pyrenoidosa contained the highest total phenol and flavonoid content (48.3 mg GAE/g extract and 34.7 mg QE/g) across all the microalgae examined in this study, whereas the lowest occurred with C. vulgaris (24.4 mg GAE/g extract and 14.0 mg QE/g). The ferric reducing antioxidant power of A. pyrenoidosa, C. vulgaris, M. gracile, D. subspicatus and P. kessleri respectively stood at 0.78, 0.62, 1.01, 0.77 and 0.68 ferrous equivalents (mM), with trolox equivalent antioxidant capacity assay revealed that A. pyrenoidosa possessed the highest scavenging activity at 1 mg/mL concentration. Interestingly, application of 1.0–2.0 × 106 cells/mL C. vulgaris, M. gracile, D. subspicatus, and P. kessleri as feed enhanced Hsp70 accumulation in Artemia, indicating that these microalgae consist stimulating compound(s) which potentially promote the synthesis of the 70-kDa stress protein in this crustacean species. Taken together, results from this study revealed that microalgae possess various antioxidant properties and coupled with their abilities to induce Hsp70, they are potential oxidative stress control alternatives in Artemia and perhaps other aquatic organisms used in aquaculture. |
| first_indexed | 2024-04-13T13:48:46Z |
| format | Article |
| id | doaj.art-415359c2016344c38a7642a5b382adc0 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-13T13:48:46Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-415359c2016344c38a7642a5b382adc02022-12-22T02:44:25ZengElsevierAquaculture Reports2352-51342020-11-0118100433Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp ArtemiaIrene Kai Ru Tiong0Thilahgavani Nagappan1Mohd Effendy Abdul Wahid2Tengku Sifzizul Tengku Muhammad3Toda Tatsuki4Woro Hastuti Satyantini5Gunanti Mahasri6Patrick Sorgeloos7Yeong Yik Sung8Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaFaculty of Science and Engineering, Soka University, 192-8577, Tokyo, JapanDepartment of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Air Langga, 60115, Surabaya, IndonesiaDepartment of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Air Langga, 60115, Surabaya, IndonesiaLaboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University, 9000, Ghent, BelgiumInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Air Langga, 60115, Surabaya, Indonesia; Corresponding author at: Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.Oxidative stress caused by chemicals brings about mass mortalities in aquatic organisms and in this context, antioxidants and heat shock protein 70 (Hsp70) stimulating compounds represent potential alternatives to alleviate this problem during aquaculture. The antioxidant capacity and efficacies of five Malaysian indigenous microalgae, namely Auxenochlorella pyrenoidosa, Chlorella vulgaris, Messastrum gracile, Desmodesmus subspicatus and Parachlorella kessleri to regulate Hsp70 expression in the brine shrimp Artemia, an important live food species, were examined. A. pyrenoidosa contained the highest total phenol and flavonoid content (48.3 mg GAE/g extract and 34.7 mg QE/g) across all the microalgae examined in this study, whereas the lowest occurred with C. vulgaris (24.4 mg GAE/g extract and 14.0 mg QE/g). The ferric reducing antioxidant power of A. pyrenoidosa, C. vulgaris, M. gracile, D. subspicatus and P. kessleri respectively stood at 0.78, 0.62, 1.01, 0.77 and 0.68 ferrous equivalents (mM), with trolox equivalent antioxidant capacity assay revealed that A. pyrenoidosa possessed the highest scavenging activity at 1 mg/mL concentration. Interestingly, application of 1.0–2.0 × 106 cells/mL C. vulgaris, M. gracile, D. subspicatus, and P. kessleri as feed enhanced Hsp70 accumulation in Artemia, indicating that these microalgae consist stimulating compound(s) which potentially promote the synthesis of the 70-kDa stress protein in this crustacean species. Taken together, results from this study revealed that microalgae possess various antioxidant properties and coupled with their abilities to induce Hsp70, they are potential oxidative stress control alternatives in Artemia and perhaps other aquatic organisms used in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513420305238AntioxidantMicroalgaeArtemia franciscanaHeat shock protein 70Stress |
| spellingShingle | Irene Kai Ru Tiong Thilahgavani Nagappan Mohd Effendy Abdul Wahid Tengku Sifzizul Tengku Muhammad Toda Tatsuki Woro Hastuti Satyantini Gunanti Mahasri Patrick Sorgeloos Yeong Yik Sung Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia Aquaculture Reports Antioxidant Microalgae Artemia franciscana Heat shock protein 70 Stress |
| title | Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia |
| title_full | Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia |
| title_fullStr | Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia |
| title_full_unstemmed | Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia |
| title_short | Antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp Artemia |
| title_sort | antioxidant capacity of five microalgae species and their effect on heat shock protein 70 expression in the brine shrimp artemia |
| topic | Antioxidant Microalgae Artemia franciscana Heat shock protein 70 Stress |
| url | http://www.sciencedirect.com/science/article/pii/S2352513420305238 |
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