Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species
Phenolic compounds were extracted from five Hypericum species (H. perforatum, H. oblongifolium, H. monogynum, H. choisianum and H. dyeri Redher) using ethanol. The crude extract (called fraction 1) was then fractionated using re-extraction to water (fraction 2), ethyl acetate (fraction 3), and aceto...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2011-01-01
|
| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/103545 |
| _version_ | 1797427860630142976 |
|---|---|
| author | Mumtaz Ali Mohammad Arfan Habib Ahmad Khair Zaman Farhatullah Khan Ryszard Amarowicz |
| author_facet | Mumtaz Ali Mohammad Arfan Habib Ahmad Khair Zaman Farhatullah Khan Ryszard Amarowicz |
| author_sort | Mumtaz Ali |
| collection | DOAJ |
| description | Phenolic compounds were extracted from five Hypericum species (H. perforatum, H. oblongifolium, H. monogynum, H. choisianum and H. dyeri Redher) using ethanol. The crude extract (called fraction 1) was then fractionated using re-extraction to water (fraction 2), ethyl acetate (fraction 3), and acetone (fraction 4). The final residue was marked as fraction 5. The content of total phenolics in the fractions ranged from 21 mg of gallic acid equivalents per g (fraction 5 of H. dyeri) to 100 mg of gallic acid equivalents per g (fraction 5 of H. choisianum). Phenolic compounds present in the fractions showed antioxidant and antiradical properties investigated using DPPH radical scavenging activity, molybdate method, and reducing power. The strongest antiradical properties were noted for fraction 3 of H. choisianum (EC50=11.2 μg/mL), whereas the weakest was for fraction 5 of H. dyeri (EC50=139.2 μg/mL). Fractions 1 and 5 of H. dyeri showed good antibacterial activity against Escherichia coli, while fractions 3–5 of H. perforatum were active against Staphylococcus aureus and Pseudomonas aeruginosa. Fractions 1–4 of H. perforatum were found most active against Helminthosporium maydis as determined by antifungal screening. |
| first_indexed | 2024-03-09T08:49:50Z |
| format | Article |
| id | doaj.art-415f637f64874bd2bf047743061992fa |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:49:50Z |
| publishDate | 2011-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-415f637f64874bd2bf047743061992fa2023-12-02T14:30:53ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062011-01-01492205213Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum SpeciesMumtaz Ali0Mohammad Arfan1Habib Ahmad2Khair Zaman3Farhatullah Khan4Ryszard Amarowicz5Department of Chemistry, University of Malakand, Chakdara, Dir, PakistanInstitute of Chemical Sciences University of Peshawar, Peshawar-25120, PakistanDepartment of Botany, Hazara University, Mansehra, PakistanInstitute of Chemical Sciences University of Peshawar, Peshawar-25120, PakistanDepartment of Biological Sciences, Quaid-e-Azam University, Islamabad, PakistanInstitute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, PolandPhenolic compounds were extracted from five Hypericum species (H. perforatum, H. oblongifolium, H. monogynum, H. choisianum and H. dyeri Redher) using ethanol. The crude extract (called fraction 1) was then fractionated using re-extraction to water (fraction 2), ethyl acetate (fraction 3), and acetone (fraction 4). The final residue was marked as fraction 5. The content of total phenolics in the fractions ranged from 21 mg of gallic acid equivalents per g (fraction 5 of H. dyeri) to 100 mg of gallic acid equivalents per g (fraction 5 of H. choisianum). Phenolic compounds present in the fractions showed antioxidant and antiradical properties investigated using DPPH radical scavenging activity, molybdate method, and reducing power. The strongest antiradical properties were noted for fraction 3 of H. choisianum (EC50=11.2 μg/mL), whereas the weakest was for fraction 5 of H. dyeri (EC50=139.2 μg/mL). Fractions 1 and 5 of H. dyeri showed good antibacterial activity against Escherichia coli, while fractions 3–5 of H. perforatum were active against Staphylococcus aureus and Pseudomonas aeruginosa. Fractions 1–4 of H. perforatum were found most active against Helminthosporium maydis as determined by antifungal screening.http://hrcak.srce.hr/file/103545Hypericum spp.phenolic compoundsantioxidant activityantimicrobial activities |
| spellingShingle | Mumtaz Ali Mohammad Arfan Habib Ahmad Khair Zaman Farhatullah Khan Ryszard Amarowicz Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species Food Technology and Biotechnology Hypericum spp. phenolic compounds antioxidant activity antimicrobial activities |
| title | Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species |
| title_full | Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species |
| title_fullStr | Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species |
| title_full_unstemmed | Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species |
| title_short | Comparative Antioxidant and Antimicrobial Activities of Phenolic Compounds Extracted from Five Hypericum Species |
| title_sort | comparative antioxidant and antimicrobial activities of phenolic compounds extracted from five hypericum species |
| topic | Hypericum spp. phenolic compounds antioxidant activity antimicrobial activities |
| url | http://hrcak.srce.hr/file/103545 |
| work_keys_str_mv | AT mumtazali comparativeantioxidantandantimicrobialactivitiesofphenoliccompoundsextractedfromfivehypericumspecies AT mohammadarfan comparativeantioxidantandantimicrobialactivitiesofphenoliccompoundsextractedfromfivehypericumspecies AT habibahmad comparativeantioxidantandantimicrobialactivitiesofphenoliccompoundsextractedfromfivehypericumspecies AT khairzaman comparativeantioxidantandantimicrobialactivitiesofphenoliccompoundsextractedfromfivehypericumspecies AT farhatullahkhan comparativeantioxidantandantimicrobialactivitiesofphenoliccompoundsextractedfromfivehypericumspecies AT ryszardamarowicz comparativeantioxidantandantimicrobialactivitiesofphenoliccompoundsextractedfromfivehypericumspecies |