Preparation and incorporation of probe-labeled apoA-I for fluorescence resonance energy transfer studies of rHDL

Preparation and incorporation of probe-labeled apoA-I for fluorescence resonance energy transfer studies of rHDL

Apolipoprotein A-I (apoA-I), the major constituent of HDL, plays an essential role in regulating cholesterol metabolism, acting as the physiological activator of lecithin: cholesterol acyltransferase, which converts cholesterol to cholesterol ester. Thiol-reactive fluorescent probes attached to cyst...

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Bibliographic Details
Main Authors: Hui-hua Li, Michael J. Thomas, Wei Pan, Eric Alexander, Michael Samuel, Mary G. Sorci-Thomas
Format: Article
Language:English
Published: Elsevier 2001-12-01
Series:Journal of Lipid Research
Subjects:
recombinant apoA-I
protein expression of mature apoA-I
fluorescent probes
Eschericha coli expression
intein
LCAT activity
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520315388

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http://www.sciencedirect.com/science/article/pii/S0022227520315388

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