Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration
Abstract Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for p...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2017-04-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0391-4 |
| _version_ | 1819242079129174016 |
|---|---|
| author | Fengling Lu Yaolin Huang Xuehong Zhang Zhilong Wang |
| author_facet | Fengling Lu Yaolin Huang Xuehong Zhang Zhilong Wang |
| author_sort | Fengling Lu |
| collection | DOAJ |
| description | Abstract Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus pigments can be optimized independently. Mycelia selected from submerged culture in a nonionic surfactant micelle aqueous solution with low pH 2.5 exhibits robust bioactivity. At the same time, enzymatic kinetic study shows that the bioactivity of mycelia as whole cell biocatalyst is sensitive to high product concentration. Segregation of product from mycelia by cell suspension culture in a nonionic surfactant micelle aqueous solution or peanut oil–water two-phase system is not only necessary for studying the enzymatic kinetics but also beneficial to industrial application of mycelia as whole cell biocatalyst. |
| first_indexed | 2024-12-23T14:34:06Z |
| format | Article |
| id | doaj.art-41815eda801d417486aae1926c166394 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-23T14:34:06Z |
| publishDate | 2017-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-41815eda801d417486aae1926c1663942022-12-21T17:43:24ZengSpringerOpenAMB Express2191-08552017-04-01711910.1186/s13568-017-0391-4Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentrationFengling Lu0Yaolin Huang1Xuehong Zhang2Zhilong Wang3School of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong UniversitySchool of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong UniversitySchool of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong UniversitySchool of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong UniversityAbstract Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus pigments can be optimized independently. Mycelia selected from submerged culture in a nonionic surfactant micelle aqueous solution with low pH 2.5 exhibits robust bioactivity. At the same time, enzymatic kinetic study shows that the bioactivity of mycelia as whole cell biocatalyst is sensitive to high product concentration. Segregation of product from mycelia by cell suspension culture in a nonionic surfactant micelle aqueous solution or peanut oil–water two-phase system is not only necessary for studying the enzymatic kinetics but also beneficial to industrial application of mycelia as whole cell biocatalyst.http://link.springer.com/article/10.1186/s13568-017-0391-4Whole cell biocatalystCultivation conditionMicrobial physiologyProduct degradation/inhibition |
| spellingShingle | Fengling Lu Yaolin Huang Xuehong Zhang Zhilong Wang Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration AMB Express Whole cell biocatalyst Cultivation condition Microbial physiology Product degradation/inhibition |
| title | Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration |
| title_full | Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration |
| title_fullStr | Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration |
| title_full_unstemmed | Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration |
| title_short | Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration |
| title_sort | biocatalytic activity of monascus mycelia depending on physiology and high sensitivity to product concentration |
| topic | Whole cell biocatalyst Cultivation condition Microbial physiology Product degradation/inhibition |
| url | http://link.springer.com/article/10.1186/s13568-017-0391-4 |
| work_keys_str_mv | AT fenglinglu biocatalyticactivityofmonascusmyceliadependingonphysiologyandhighsensitivitytoproductconcentration AT yaolinhuang biocatalyticactivityofmonascusmyceliadependingonphysiologyandhighsensitivitytoproductconcentration AT xuehongzhang biocatalyticactivityofmonascusmyceliadependingonphysiologyandhighsensitivitytoproductconcentration AT zhilongwang biocatalyticactivityofmonascusmyceliadependingonphysiologyandhighsensitivitytoproductconcentration |