Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products

Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products

Abstract Objective The purpose of this project was to use an in vivo method to discover riboswitches that are activated by new ligands. We employed phage-assisted continuous evolution (PACE) to evolve new riboswitches in vivo. We started with one translational riboswitch and one transcriptional ribo...

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Bibliographic Details
Main Authors: Zachary M. Shaver, Stephanie S. Bent, Steven R. Bilby, Michael Brown, Anna Buser, Itzayana G. Cuellar, Athena J. Davis, Lindsay Doolan, Fatima C. Enriquez, Autumn Estrada, Shelby Herner, J. Cody Herron, Andrew M. Hunn, Madison Hunter, Hartlee Johnston, Owen Koucky, Christian C. Mackley, Dylan Maghini, Devin Mattoon, Haden T. McDonald, Hannah Sinks, Austin J. Sprague, David Sullivan, Altan Tutar, Avery Umphreys, Chris Watson, Daniel Zweerink, Laurie J. Heyer, Jeffrey L. Poet, Todd T. Eckdahl, A. Malcolm Campbell
Format: Article
Language:English
Published: BMC 2018-12-01
Series:BMC Research Notes
Subjects:
Phage-assisted continuous evolution (PACE)
Riboswitch
Synthetic biology
Metabolic engineering
Theophylline
Xanthine
Online Access:http://link.springer.com/article/10.1186/s13104-018-3965-6

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http://link.springer.com/article/10.1186/s13104-018-3965-6

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