Introduction of a multiplex amplicon sequencing assay to quantify DNA methylation in target cytosine markers underlying four selected epigenetic clocks

Introduction of a multiplex amplicon sequencing assay to quantify DNA methylation in target cytosine markers underlying four selected epigenetic clocks

Abstract Background DNA methylation analysis has proven to be a powerful tool for age assessment. However, the implementation of epigenetic age prediction in diagnostics or routine forensic casework requires appropriate laboratory methods. In this study, we aimed to compare the performance of large-...

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Bibliographic Details
Main Authors: Ewelina Pośpiech, Aleksandra Pisarek, Joanna Rudnicka, Rezvan Noroozi, Michał Boroń, Aleksander Masny, Bożena Wysocka, Kamila Migacz-Gruszka, Dagmara Lisman, Paulina Pruszkowska-Przybylska, Magdalena Kobus, Maria Szargut, Joanna Dowejko, Kamila Stanisz, Julia Zacharczuk, Piotr Zieliński, Aneta Sitek, Andrzej Ossowski, Magdalena Spólnicka, Wojciech Branicki
Format: Article
Language:English
Published: BMC 2023-08-01
Series:Clinical Epigenetics
Subjects:
DNA methylation analysis methods
High-throughput sequencing
Target enrichment protocols
Epigenetic age estimation
Pace of aging
Mortality risk score
Online Access:https://doi.org/10.1186/s13148-023-01545-2

Internet

https://doi.org/10.1186/s13148-023-01545-2

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