Biotin controls intestinal stem cell mitosis and host-microbiome interactions
Summary: Diet is a key regulator of metabolism and interacts with the intestinal microbiome. Here, we study the role of the Drosophila intestinal stem cell (ISC)-specific biotin transporter Smvt in midgut homeostasis, infection-induced regeneration, and tumorigenesis. We show that Smvt-transported b...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124722002418 |
| _version_ | 1819349513513467904 |
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| author | Constantina Neophytou Chrysoula Pitsouli |
| author_facet | Constantina Neophytou Chrysoula Pitsouli |
| author_sort | Constantina Neophytou |
| collection | DOAJ |
| description | Summary: Diet is a key regulator of metabolism and interacts with the intestinal microbiome. Here, we study the role of the Drosophila intestinal stem cell (ISC)-specific biotin transporter Smvt in midgut homeostasis, infection-induced regeneration, and tumorigenesis. We show that Smvt-transported biotin in ISCs is necessary for ISC mitosis. Smvt deficiency impairs intestinal maintenance, which can be rescued by the human Smvt, encoded by SLC5A6. ISC-specific, Smvt-silenced flies exhibit microbial dysbiosis, whereby the growth of Providencia sneebia, an opportunistic pathogen, is favored. Dysbiosis correlates with increased Nox expression, reactive oxygen species (ROS), and enterocyte apoptosis. Flies acquire biotin from their diet and microbiota. We show that, when dietary biotin is scarce, biotin-producing commensals, e.g., E. coli, can rescue reduced ISC mitosis. Smvt and commensals also control intestinal tumor growth. Our findings suggest that direct modification of the gut microbiome by biotin can serve as an approach for the treatment of dysbiosis-promoted diseases and tumorigenesis control. |
| first_indexed | 2024-12-24T19:01:43Z |
| format | Article |
| id | doaj.art-41afa9757fa04298a2e55886b6a7161e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-24T19:01:43Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-41afa9757fa04298a2e55886b6a7161e2022-12-21T16:43:11ZengElsevierCell Reports2211-12472022-03-013810110505Biotin controls intestinal stem cell mitosis and host-microbiome interactionsConstantina Neophytou0Chrysoula Pitsouli1Department of Biological Sciences, University of Cyprus, 1 University Avenue, Aglantzia 2109, CyprusDepartment of Biological Sciences, University of Cyprus, 1 University Avenue, Aglantzia 2109, Cyprus; Corresponding authorSummary: Diet is a key regulator of metabolism and interacts with the intestinal microbiome. Here, we study the role of the Drosophila intestinal stem cell (ISC)-specific biotin transporter Smvt in midgut homeostasis, infection-induced regeneration, and tumorigenesis. We show that Smvt-transported biotin in ISCs is necessary for ISC mitosis. Smvt deficiency impairs intestinal maintenance, which can be rescued by the human Smvt, encoded by SLC5A6. ISC-specific, Smvt-silenced flies exhibit microbial dysbiosis, whereby the growth of Providencia sneebia, an opportunistic pathogen, is favored. Dysbiosis correlates with increased Nox expression, reactive oxygen species (ROS), and enterocyte apoptosis. Flies acquire biotin from their diet and microbiota. We show that, when dietary biotin is scarce, biotin-producing commensals, e.g., E. coli, can rescue reduced ISC mitosis. Smvt and commensals also control intestinal tumor growth. Our findings suggest that direct modification of the gut microbiome by biotin can serve as an approach for the treatment of dysbiosis-promoted diseases and tumorigenesis control.http://www.sciencedirect.com/science/article/pii/S2211124722002418dysbiosisbiotinSmvtSLC5A6DrosophilaProvidencia sneebia |
| spellingShingle | Constantina Neophytou Chrysoula Pitsouli Biotin controls intestinal stem cell mitosis and host-microbiome interactions Cell Reports dysbiosis biotin Smvt SLC5A6 Drosophila Providencia sneebia |
| title | Biotin controls intestinal stem cell mitosis and host-microbiome interactions |
| title_full | Biotin controls intestinal stem cell mitosis and host-microbiome interactions |
| title_fullStr | Biotin controls intestinal stem cell mitosis and host-microbiome interactions |
| title_full_unstemmed | Biotin controls intestinal stem cell mitosis and host-microbiome interactions |
| title_short | Biotin controls intestinal stem cell mitosis and host-microbiome interactions |
| title_sort | biotin controls intestinal stem cell mitosis and host microbiome interactions |
| topic | dysbiosis biotin Smvt SLC5A6 Drosophila Providencia sneebia |
| url | http://www.sciencedirect.com/science/article/pii/S2211124722002418 |
| work_keys_str_mv | AT constantinaneophytou biotincontrolsintestinalstemcellmitosisandhostmicrobiomeinteractions AT chrysoulapitsouli biotincontrolsintestinalstemcellmitosisandhostmicrobiomeinteractions |