Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes

Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes

Summary: Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell f...

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Bibliographic Details
Main Authors: Irene Ganan-Gomez, Karen Clise-Dwyer, Simona Colla
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Cell separation/fractionation
Cancer
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200644X

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http://www.sciencedirect.com/science/article/pii/S266616672200644X

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