| Summary: | The aim of the study was to evaluate the fungicidal effect of a H<sub>2</sub>O<sub>2</sub> mist generating system for disinfection of spores of six food-related moulds (<i>Alternaria alternata</i>, <i>Aspergillus flavus</i>, <i>Geotrichum candidum</i>, <i>Mucor plumbeus</i>, <i>Paecilomyces variotii</i>, and <i>Penicillium solitum</i>) dried on stainless steel. Exposure to H<sub>2</sub>O<sub>2</sub> mist for 2 or 4 h lead to >3 log reduction in mould spores in the majority of the tests. The presence of the soils 2% skim milk or 3% BSA did not significantly alter the fungicidal effect, while the presence of raw meat juice had an adverse fungicidal effect against <i>Penicillium</i> and <i>Mucor</i> in two out of three tests. Fungicidal suspension tests with liquid H<sub>2</sub>O<sub>2</sub> confirmed the effectiveness of H<sub>2</sub>O<sub>2</sub> on reducing the mould spores. Both the surface test and the suspension test indicated that <i>P. variotii</i> is more resistant to H<sub>2</sub>O<sub>2</sub> compared to the other moulds tested. The study shows the efficiency of H<sub>2</sub>O<sub>2</sub> mist on reducing food-related mould spores on surfaces.
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