Generation of precision-cut slice cultures of human placenta

Summary: We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular...

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Bibliographic Details
Main Authors: Amal Fahmi, Melanie Brügger, Beatrice Zumkehr, Blandina I. Oliveira Esteves, David Baud, Marco P. Alves
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200171X