In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants
Stevia a „Latin American herb‟ is the world‟s only natural sweetener with zero calories, zero carbohydrates and a zero glycemic index. In the present investigation, the in vitro regeneration of Stevia rebaudiana was performed through callogenesis and organogenesis from different explants (Leaves, In...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2012-09-01
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| Series: | Electronic Journal of Plant Breeding |
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| Online Access: | https://sites.google.com/site/ejpb2011/vol-3-3/Vol-3-3-916-924.pdf?attredirects=0 |
| _version_ | 1818412848692330496 |
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| author | Arvind Arya, Sandeep Kumar, and M.S. Kasana |
| author_facet | Arvind Arya, Sandeep Kumar, and M.S. Kasana |
| author_sort | Arvind Arya, Sandeep Kumar, and M.S. Kasana |
| collection | DOAJ |
| description | Stevia a „Latin American herb‟ is the world‟s only natural sweetener with zero calories, zero carbohydrates and a zero glycemic index. In the present investigation, the in vitro regeneration of Stevia rebaudiana was performed through callogenesis and organogenesis from different explants (Leaves, Inter node, Shoot discs). Leaves explants showed best callus induction response when cultured on MS + (2.0 μM BAP + 1.0 μM NAA). However, the best callus initiation from leaf explants was obtained on MS medium supplemented with 1.0 μM NAA + 1.0 μM Kn. Optimization results of medium type and carbon source confirm 1X MS medium and glucose (3%) respectively best for callus initiation. Shoot initiation was achieved on 1X MS medium supplemented with 5.0 μM BAP + 1.0 μM NAA. In vitro raised shoots of Stevia showed best rooting on 1X MS + (1.0 μM IAA). The methanolic extracts of regenerated plants and callus culture of Stevia showed best antibacterial and antifungal activity against a number of microorganisms. Antiprotozoal activity of methanolic extract was also tested and found satisfactory. In vitro regeneration protocol of Stevia through callus culture is advantageous for enhanced multiplication of superior Stevia cultivars. Furthermore, callus from leaf explants can be a good source for production of antimicrobial and antiprotozoal compound of Stevia through bioreactor. |
| first_indexed | 2024-12-14T10:53:50Z |
| format | Article |
| id | doaj.art-4232cefad1744d35b588ae62db4005e7 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-12-14T10:53:50Z |
| publishDate | 2012-09-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-4232cefad1744d35b588ae62db4005e72022-12-21T23:05:03ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2012-09-0133916924In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plantsArvind Arya, Sandeep Kumar, and M.S. KasanaStevia a „Latin American herb‟ is the world‟s only natural sweetener with zero calories, zero carbohydrates and a zero glycemic index. In the present investigation, the in vitro regeneration of Stevia rebaudiana was performed through callogenesis and organogenesis from different explants (Leaves, Inter node, Shoot discs). Leaves explants showed best callus induction response when cultured on MS + (2.0 μM BAP + 1.0 μM NAA). However, the best callus initiation from leaf explants was obtained on MS medium supplemented with 1.0 μM NAA + 1.0 μM Kn. Optimization results of medium type and carbon source confirm 1X MS medium and glucose (3%) respectively best for callus initiation. Shoot initiation was achieved on 1X MS medium supplemented with 5.0 μM BAP + 1.0 μM NAA. In vitro raised shoots of Stevia showed best rooting on 1X MS + (1.0 μM IAA). The methanolic extracts of regenerated plants and callus culture of Stevia showed best antibacterial and antifungal activity against a number of microorganisms. Antiprotozoal activity of methanolic extract was also tested and found satisfactory. In vitro regeneration protocol of Stevia through callus culture is advantageous for enhanced multiplication of superior Stevia cultivars. Furthermore, callus from leaf explants can be a good source for production of antimicrobial and antiprotozoal compound of Stevia through bioreactor.https://sites.google.com/site/ejpb2011/vol-3-3/Vol-3-3-916-924.pdf?attredirects=0AntibacterialAntifungalAntiplasmodialAntiprotozoalAuxin |
| spellingShingle | Arvind Arya, Sandeep Kumar, and M.S. Kasana In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants Electronic Journal of Plant Breeding Antibacterial Antifungal Antiplasmodial Antiprotozoal Auxin |
| title | In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants |
| title_full | In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants |
| title_fullStr | In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants |
| title_full_unstemmed | In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants |
| title_short | In vitro regeneration of Stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants |
| title_sort | in vitro regeneration of stevia and evaluation of antimicrobial and antiprotozoal properties of regenerated calli and plants |
| topic | Antibacterial Antifungal Antiplasmodial Antiprotozoal Auxin |
| url | https://sites.google.com/site/ejpb2011/vol-3-3/Vol-3-3-916-924.pdf?attredirects=0 |
| work_keys_str_mv | AT arvindaryasandeepkumarandmskasana invitroregenerationofsteviaandevaluationofantimicrobialandantiprotozoalpropertiesofregeneratedcalliandplants |