A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen

A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen

Aims: The well-documented relationship between sperm oxidation and male infertility strongly encourages the development of assays for reactive oxygen species detection in semen samples. The present study aims to apply the microplate-based 2’,7’-dichlorofluorescein diacetate assay to the evaluation o...

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Bibliographic Details
Main Authors: Serena Benedetti, Simona Catalani, Silvia De Stefani, Mariangela Primiterra, Alessandra Fraternale, Francesco Palma, Simone Palini
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Heliyon
Subjects:
2’,7’-dichlorofluorescein diacetate
Male infertility
Microplate fluorescence reader
Oxidative stress
Pro-glutathione molecules
Reactive oxygen species
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844022019302

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http://www.sciencedirect.com/science/article/pii/S2405844022019302

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