High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent

Gene manipulation in non-adhesive cells, especially lymphocytes, was difficult due to their low efficiency and high toxicity. Electroporation was reported as a highly efficient method for human and mouse lymphocytes. However, this method requires expensive equipment and causes severe cell damage. He...

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Bibliographic Details
Main Authors: Song Wang, Dan Tian
Format: Article
Language:English
Published: Taylor & Francis Group 2022-05-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0024