High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent
Gene manipulation in non-adhesive cells, especially lymphocytes, was difficult due to their low efficiency and high toxicity. Electroporation was reported as a highly efficient method for human and mouse lymphocytes. However, this method requires expensive equipment and causes severe cell damage. He...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2022-05-01
|
Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0024 |