An end-to-end approach for single-cell infrared absorption spectroscopy of bacterial inclusion bodies: from AFM-IR measurement to data interpretation of large sample sets

An end-to-end approach for single-cell infrared absorption spectroscopy of bacterial inclusion bodies: from AFM-IR measurement to data interpretation of large sample sets

Abstract Background Inclusion bodies (IBs) are well-known subcellular structures in bacteria where protein aggregates are collected. Various methods have probed their structure, but single-cell spectroscopy remains challenging. Atomic Force Microscopy-based Infrared Spectroscopy (AFM-IR) is a novel...

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Bibliographic Details
Main Authors: Wouter Duverger, Grigoria Tsaka, Ladan Khodaparast, Laleh Khodaparast, Nikolaos Louros, Frederic Rousseau, Joost Schymkowitz
Format: Article
Language:English
Published: BMC 2024-07-01
Series:Journal of Nanobiotechnology
Subjects:
AFM-IR
Image analysis
Protein aggregation
Escherichia coli
Infrared spectroscopy
Online Access:https://doi.org/10.1186/s12951-024-02674-3

Internet

https://doi.org/10.1186/s12951-024-02674-3

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