Characterization of Intact Neo-Glycoproteins by Hydrophilic Interaction Liquid Chromatography

Characterization of Intact Neo-Glycoproteins by Hydrophilic Interaction Liquid Chromatography

In this study, an HPLC HILIC-UV method was developed for the analysis of intact neo-glycoproteins. During method development the experimental conditions evaluated involved different HILIC columns (TSKgel Amide-80 and ZIC-pHILIC), and water-acetonitrile mixtures containing various types of acids and...

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Bibliographic Details
Main Authors: Alice Pedrali, Sara Tengattini, Giorgio Marrubini, Teodora Bavaro, Petrus Hemström, Gabriella Massolini, Marco Terreni, Caterina Temporini
Format: Article
Language:English
Published: MDPI AG 2014-06-01
Series:Molecules
Subjects:
hydrophilic interaction liquid chromatography
intact glycoproteins
semi-synthetic glycosylation monitoring
neo-glycoproteins
Online Access:http://www.mdpi.com/1420-3049/19/7/9070

Internet

http://www.mdpi.com/1420-3049/19/7/9070

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