Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification
Abstract Objective Comprehensive and reliable genome-wide variant analysis of a small number of cells has been challenging due to genome coverage bias, PCR over-cycling, and the requirement of expensive technologies. To comprehensively identify genome alterations in single colon crypts that reflect...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-04-01
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Series: | BMC Research Notes |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13104-023-06333-y |