Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification

Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification

Abstract Objective Comprehensive and reliable genome-wide variant analysis of a small number of cells has been challenging due to genome coverage bias, PCR over-cycling, and the requirement of expensive technologies. To comprehensively identify genome alterations in single colon crypts that reflect...

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Bibliographic Details
Main Authors: Zarko Manojlovic, Jordan Wlodarczyk, Cindy Okitsu, Yuxin Jin, David Van Den Berg, Michael R. Lieber, Chih-Lin Hsieh
Format: Article
Language:English
Published: BMC 2023-04-01
Series:BMC Research Notes
Subjects:
Next generation sequencing
High coverage whole-genome sequencing
Sequencing library construction without DNA extraction
Online Access:https://doi.org/10.1186/s13104-023-06333-y

Internet

https://doi.org/10.1186/s13104-023-06333-y

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