Efficient Gene Transfection by Electroporation—In Vitro and In Silico Study of Pulse Parameters

Gene electrotransfer (GET) is a widely used method for nucleic acids’ delivery into cells. We explored, evaluated, and demonstrated the potential use of different pulse durations for introducing plasmid DNA (pDNA) into cells in vitro and compared the efficiency and dynamics of transgene expression a...

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Bibliographic Details
Main Authors: Tjaša Potočnik, Shaurya Sachdev, Tamara Polajžer, Alenka Maček Lebar, Damijan Miklavčič
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/12/16/8237