Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories
Abstract Purpose Yeasts are widely used for the production of bioethanol from biomasses rich in sugar. The present study was aimed at isolating, screening, and characterizing fermentative wild yeast recovered from bio-waste and co-products of Ethiopian sugar factories for bioethanol production using...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | Annals of Microbiology |
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| Online Access: | https://doi.org/10.1186/s13213-022-01695-3 |
| _version_ | 1818026139381137408 |
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| author | Estifanos Hawaz Mesfin Tafesse Anteneh Tesfaye Dereje Beyene Solomon Kiros Gessese Kebede Teun Boekhout Bart Theelen Marizeth Groenewald Ayantu Degefe Sissay Degu Alene Admas Diriba Muleta |
| author_facet | Estifanos Hawaz Mesfin Tafesse Anteneh Tesfaye Dereje Beyene Solomon Kiros Gessese Kebede Teun Boekhout Bart Theelen Marizeth Groenewald Ayantu Degefe Sissay Degu Alene Admas Diriba Muleta |
| author_sort | Estifanos Hawaz |
| collection | DOAJ |
| description | Abstract Purpose Yeasts are widely used for the production of bioethanol from biomasses rich in sugar. The present study was aimed at isolating, screening, and characterizing fermentative wild yeast recovered from bio-waste and co-products of Ethiopian sugar factories for bioethanol production using sugarcane molasses as a substrate. Method The wild yeasts were identified according to their cellular morphology and D1/D2 and ITS1-5.8S-ITS2 rDNA sequencing. Analysis of ethanol and by-product concentration was done by HPLC equipped with a UV detector. Higher alcohols, acetaldehyde, and methanol were analyzed using GC-MS equipped with a flame ionization detector (FID). Result Seven strains (Meyerozyma caribbica MJTm3, Meyerozyma caribbica MJTPm4, Meyerozyma caribbica SHJF, Saccharomyces cerevisiae TA2, Wickerhamomyces anomalus MJTPm2, Wickerhamomyces anomalus 4m10, and Wickerhamomyces anomalus HCJ2F) were found tolerant to 18% (v/v) ethanol, whereas one strain Meyerozyma caribbica MJTm3 tolerated 20%. These strains also showed tolerance to 45°C, 50% of sugar, and pH 2–10. Meyerozyma caribbica MJTm3 produced 12.7% (v/v) of alcohol with an actual ethanol concentration of 26 g L−1, an ethanol yield of 47%, 78% of theoretical yield, and a productivity of 0.54 g L−1 h−1 from 30 °Brix of molasses at 48 h incubation under laboratory scale. Based on the one variable at a time optimization (OVAT), the optimal parameters for maximum bioethanol production were at initial pH 5.5, 35 °Brix, 30°C, 15% inoculum size, 150 rpm, 4 g L−1 di-ammonium phosphate supplement, and 48 h incubation. Under these optimum conditions, 14% (v/v) alcohol, 42 g L−1 actual ethanol concentration, 69% ethanol yield, 89% of theoretical yield, and productivity of 0.88 g L−1 h−1 were obtained. Conclusion These results indicated that M. caribbica MJTm3 should further be evaluated, optimized, and improved for industrial bioethanol production due to its fermentation potential. |
| first_indexed | 2024-12-10T04:27:15Z |
| format | Article |
| id | doaj.art-4286e7a0dbe7404b897fa6121c7e657c |
| institution | Directory Open Access Journal |
| issn | 1590-4261 1869-2044 |
| language | English |
| last_indexed | 2024-12-10T04:27:15Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Microbiology |
| spelling | doaj.art-4286e7a0dbe7404b897fa6121c7e657c2022-12-22T02:02:15ZengBMCAnnals of Microbiology1590-42611869-20442022-09-0172111610.1186/s13213-022-01695-3Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factoriesEstifanos Hawaz0Mesfin Tafesse1Anteneh Tesfaye2Dereje Beyene3Solomon Kiros4Gessese Kebede5Teun Boekhout6Bart Theelen7Marizeth Groenewald8Ayantu Degefe9Sissay Degu10Alene Admas11Diriba Muleta12Institute of Biotechnology, Addis Ababa UniversityCenter of Excellence for Biotechnology and Bioprocess, Addis Ababa Science and Technology UniversityInstitute of Biotechnology, Addis Ababa UniversityDepartment of Microbial, Cellular and Molecular Biology, Addis Ababa UniversityInstitute of Technology, Addis Ababa UniversityCenter of Excellence for Biotechnology and Bioprocess, Addis Ababa Science and Technology UniversityWesterdijk Fungal Biodiversity InstituteWesterdijk Fungal Biodiversity InstituteWesterdijk Fungal Biodiversity InstituteInstitute of Biotechnology, Addis Ababa UniversityInstitute of Biotechnology, Addis Ababa UniversityInstitute of Technology, Addis Ababa UniversityInstitute of Biotechnology, Addis Ababa UniversityAbstract Purpose Yeasts are widely used for the production of bioethanol from biomasses rich in sugar. The present study was aimed at isolating, screening, and characterizing fermentative wild yeast recovered from bio-waste and co-products of Ethiopian sugar factories for bioethanol production using sugarcane molasses as a substrate. Method The wild yeasts were identified according to their cellular morphology and D1/D2 and ITS1-5.8S-ITS2 rDNA sequencing. Analysis of ethanol and by-product concentration was done by HPLC equipped with a UV detector. Higher alcohols, acetaldehyde, and methanol were analyzed using GC-MS equipped with a flame ionization detector (FID). Result Seven strains (Meyerozyma caribbica MJTm3, Meyerozyma caribbica MJTPm4, Meyerozyma caribbica SHJF, Saccharomyces cerevisiae TA2, Wickerhamomyces anomalus MJTPm2, Wickerhamomyces anomalus 4m10, and Wickerhamomyces anomalus HCJ2F) were found tolerant to 18% (v/v) ethanol, whereas one strain Meyerozyma caribbica MJTm3 tolerated 20%. These strains also showed tolerance to 45°C, 50% of sugar, and pH 2–10. Meyerozyma caribbica MJTm3 produced 12.7% (v/v) of alcohol with an actual ethanol concentration of 26 g L−1, an ethanol yield of 47%, 78% of theoretical yield, and a productivity of 0.54 g L−1 h−1 from 30 °Brix of molasses at 48 h incubation under laboratory scale. Based on the one variable at a time optimization (OVAT), the optimal parameters for maximum bioethanol production were at initial pH 5.5, 35 °Brix, 30°C, 15% inoculum size, 150 rpm, 4 g L−1 di-ammonium phosphate supplement, and 48 h incubation. Under these optimum conditions, 14% (v/v) alcohol, 42 g L−1 actual ethanol concentration, 69% ethanol yield, 89% of theoretical yield, and productivity of 0.88 g L−1 h−1 were obtained. Conclusion These results indicated that M. caribbica MJTm3 should further be evaluated, optimized, and improved for industrial bioethanol production due to its fermentation potential.https://doi.org/10.1186/s13213-022-01695-3BioethanolFermentationMolassesOptimizationStress toleranceWild yeast |
| spellingShingle | Estifanos Hawaz Mesfin Tafesse Anteneh Tesfaye Dereje Beyene Solomon Kiros Gessese Kebede Teun Boekhout Bart Theelen Marizeth Groenewald Ayantu Degefe Sissay Degu Alene Admas Diriba Muleta Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories Annals of Microbiology Bioethanol Fermentation Molasses Optimization Stress tolerance Wild yeast |
| title | Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories |
| title_full | Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories |
| title_fullStr | Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories |
| title_full_unstemmed | Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories |
| title_short | Isolation and characterization of bioethanol producing wild yeasts from bio-wastes and co-products of sugar factories |
| title_sort | isolation and characterization of bioethanol producing wild yeasts from bio wastes and co products of sugar factories |
| topic | Bioethanol Fermentation Molasses Optimization Stress tolerance Wild yeast |
| url | https://doi.org/10.1186/s13213-022-01695-3 |
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