Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats
Oxidative stress and inflammation are well-known triggers of NAFLD onset and progression. The aim of this study is to compare the potential benefits of a viable probiotic (<i>Lactobacillus rhamnosus</i> GG) and its parabiotic (heat-inactivated) on oxidative stress, inflammation, DNA dama...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/3/717 |
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| author | Laura Arellano-García Jenifer Trepiana J. Alfredo Martínez María P. Portillo Iñaki Milton-Laskibar |
| author_facet | Laura Arellano-García Jenifer Trepiana J. Alfredo Martínez María P. Portillo Iñaki Milton-Laskibar |
| author_sort | Laura Arellano-García |
| collection | DOAJ |
| description | Oxidative stress and inflammation are well-known triggers of NAFLD onset and progression. The aim of this study is to compare the potential benefits of a viable probiotic (<i>Lactobacillus rhamnosus</i> GG) and its parabiotic (heat-inactivated) on oxidative stress, inflammation, DNA damage and cell death pathways in the liver of rats featuring diet-induced NAFLD. The consumption of the steatotic diet led to increased final body and liver weights, higher hepatic triacylglycerol content, altered serum transaminase levels and enhanced oxidative and inflammatory status. Administration of the probiotic and the parabiotic partially prevented the body weight increase induced by the steatotic diet, whereas the probiotic caused more effective decreasing hepatic triglyceride content. Sharp but nonstatistically significant decreases in serum transaminase levels were also observed for both treatments. The reduction in antioxidant enzyme activities found in the nontreated animals fed the steatotic diet was partially prevented by both treatments (GPx activity). Similarly, the reductions in nonenzymatic antioxidant protection (GSH content) and total antioxidant capacity (ORAC) found in the nontreated rats were restored by the administration of both treatments. These results show that both viable and heat-inactivated <i>Lactobacillus rhamnosus</i> GG administration partially prevent steatotic diet-induced liver oxidative stress and inflammation induced in rats. |
| first_indexed | 2024-03-11T07:00:23Z |
| format | Article |
| id | doaj.art-42aaad98d00f40a5af6e505eb1781fcb |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T07:00:23Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-42aaad98d00f40a5af6e505eb1781fcb2023-11-17T09:18:47ZengMDPI AGAntioxidants2076-39212023-03-0112371710.3390/antiox12030717Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in RatsLaura Arellano-García0Jenifer Trepiana1J. Alfredo Martínez2María P. Portillo3Iñaki Milton-Laskibar4Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Centre, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Centre, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, SpainCIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, 28222 Madrid, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Centre, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Centre, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, SpainOxidative stress and inflammation are well-known triggers of NAFLD onset and progression. The aim of this study is to compare the potential benefits of a viable probiotic (<i>Lactobacillus rhamnosus</i> GG) and its parabiotic (heat-inactivated) on oxidative stress, inflammation, DNA damage and cell death pathways in the liver of rats featuring diet-induced NAFLD. The consumption of the steatotic diet led to increased final body and liver weights, higher hepatic triacylglycerol content, altered serum transaminase levels and enhanced oxidative and inflammatory status. Administration of the probiotic and the parabiotic partially prevented the body weight increase induced by the steatotic diet, whereas the probiotic caused more effective decreasing hepatic triglyceride content. Sharp but nonstatistically significant decreases in serum transaminase levels were also observed for both treatments. The reduction in antioxidant enzyme activities found in the nontreated animals fed the steatotic diet was partially prevented by both treatments (GPx activity). Similarly, the reductions in nonenzymatic antioxidant protection (GSH content) and total antioxidant capacity (ORAC) found in the nontreated rats were restored by the administration of both treatments. These results show that both viable and heat-inactivated <i>Lactobacillus rhamnosus</i> GG administration partially prevent steatotic diet-induced liver oxidative stress and inflammation induced in rats.https://www.mdpi.com/2076-3921/12/3/717NAFLDliver steatosisprobioticsparabiotics<i>Lactobacillus rhamnosus</i> GGoxidative stress |
| spellingShingle | Laura Arellano-García Jenifer Trepiana J. Alfredo Martínez María P. Portillo Iñaki Milton-Laskibar Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats Antioxidants NAFLD liver steatosis probiotics parabiotics <i>Lactobacillus rhamnosus</i> GG oxidative stress |
| title | Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats |
| title_full | Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats |
| title_fullStr | Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats |
| title_full_unstemmed | Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats |
| title_short | Beneficial Effects of Viable and Heat-Inactivated <i>Lactobacillus rhamnosus</i> GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats |
| title_sort | beneficial effects of viable and heat inactivated i lactobacillus rhamnosus i gg administration on oxidative stress and inflammation in diet induced nafld in rats |
| topic | NAFLD liver steatosis probiotics parabiotics <i>Lactobacillus rhamnosus</i> GG oxidative stress |
| url | https://www.mdpi.com/2076-3921/12/3/717 |
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