Monolithic Papain-Immobilized Enzyme Reactors for Automated Structural Characterization of Monoclonal Antibodies

Monolithic Papain-Immobilized Enzyme Reactors for Automated Structural Characterization of Monoclonal Antibodies

The characterization of monoclonal antibodies (mAbs) requires laborious and time-consuming sample preparation steps before the liquid chromatography–mass spectrometry (LC-MS) analysis. Middle-up approaches entailing the use of specific proteases (papain, IdeS, etc.) emerged as practical and informat...

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Bibliographic Details
Main Authors: Francesca Rinaldi, Sara Tengattini, Gloria Brusotti, Giuseppe Tripodo, Benjamin Peters, Caterina Temporini, Gabriella Massolini, Enrica Calleri
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Molecular Biosciences
Subjects:
monoclonal antibodies
analytical characterization
immobilized enzyme reactor (IMER)
monolithic supports
polymerized high internal phase emulsions (polyHIPEs)
papain
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.765683/full

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https://www.frontiersin.org/articles/10.3389/fmolb.2021.765683/full

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