qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

Abstract Background Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-established method for analysing gene expression. Most RT-qPCR experiments in the field of microbiology aim for the detection of transcriptional changes by relative quantification, which means the comparison of...

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Bibliographic Details
Main Authors: Daniel Flatschacher, Verena Speckbacher, Susanne Zeilinger
Format: Article
Language:English
Published: BMC 2022-07-01
Series:BMC Bioinformatics
Subjects:
Reverse transcription quantitative real-time (RT-qPCR )
Shiny
Relative quantification
Relative expression analysis
Fold change
R
Online Access:https://doi.org/10.1186/s12859-022-04823-7

Internet

https://doi.org/10.1186/s12859-022-04823-7

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