Detection of exogenous DNA uptake by murine dendritic cells

Detection of exogenous DNA uptake by murine dendritic cells

Summary: This protocol has been developed to measure exogenous DNA uptake by murine dendritic cells (DCs) using supernatant containing cellular debris, which allows for DNA uptake in the absence of transfection reagents. Inhibitors or antibodies that alter the process can be added, and either flow c...

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Bibliographic Details
Main Authors: Daiana P. Celias, Álvaro de Mingo Pulido, Brian Ruffell
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cancer
Cell Biology
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003446

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http://www.sciencedirect.com/science/article/pii/S2666166722003446

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