Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase

The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur. It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs). Impr...

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Bibliographic Details
Main Authors: Jia-Hao Wang, Su-Jun Wu, Yan Li, Yue Zhao, Zhi-Mei Liu, Shou-Long Deng, Zheng-Xing Lian
Format: Article
Language:English
Published: MDPI AG 2023-07-01
Series:Cells
Subjects:
Online Access:https://www.mdpi.com/2073-4409/12/14/1818