Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020
ObjectiveTo prepare proficiency testing samples of Cronobacter spp. and apply to laboratory test in 2018-2020.MethodsThe background bacteria and Cronobacter spp. were identified by biochemical reaction and MALDI-TOF MS. Cronobacter spp. negative samples (background bacteria only) and positive sample...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2022-01-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20220106?st=article_issue |
| _version_ | 1797976220143452160 |
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| author | LIU Na ZHAO Linna WANG Xueshuo CUI Shenghui |
| author_facet | LIU Na ZHAO Linna WANG Xueshuo CUI Shenghui |
| author_sort | LIU Na |
| collection | DOAJ |
| description | ObjectiveTo prepare proficiency testing samples of Cronobacter spp. and apply to laboratory test in 2018-2020.MethodsThe background bacteria and Cronobacter spp. were identified by biochemical reaction and MALDI-TOF MS. Cronobacter spp. negative samples (background bacteria only) and positive samples (background bacteria and Cronobacter spp.) were prepared by freeze-drying. 20 samples were selected randomly for uniformity test. The samples were stored respectively at -20 ℃ and 4 ℃ to evaluate the storage stability, at 25 ℃ and 37 ℃ for 7 d to evaluate the transport stability. Samples were distributed to laboratories participating in the proficiency with operation instruction, and the feedback result from laboratories were analyzed.ResultsThe identified result of all the related strains were correct. Both of positive samples and negative samples were uniform and stable. The satisfaction rates of assessment result were 97.1%, 94.7% and 100% respectively in 2018-2020.ConclusionThe samples could meet the requirements of proficiency test. By organization proficiency test, the difference of laboratories can be reflected which is helpful to further improve the laboratory test capability. |
| first_indexed | 2024-04-11T04:47:37Z |
| format | Article |
| id | doaj.art-432b92656228421896ba282af70334c6 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:47:37Z |
| publishDate | 2022-01-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-432b92656228421896ba282af70334c62022-12-27T09:24:30ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562022-01-013401293310.13590/j.cjfh.2022.01.0061004-8456(2022)01-0029-05Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020LIU NaZHAO LinnaWANG XueshuoCUI ShenghuiObjectiveTo prepare proficiency testing samples of Cronobacter spp. and apply to laboratory test in 2018-2020.MethodsThe background bacteria and Cronobacter spp. were identified by biochemical reaction and MALDI-TOF MS. Cronobacter spp. negative samples (background bacteria only) and positive samples (background bacteria and Cronobacter spp.) were prepared by freeze-drying. 20 samples were selected randomly for uniformity test. The samples were stored respectively at -20 ℃ and 4 ℃ to evaluate the storage stability, at 25 ℃ and 37 ℃ for 7 d to evaluate the transport stability. Samples were distributed to laboratories participating in the proficiency with operation instruction, and the feedback result from laboratories were analyzed.ResultsThe identified result of all the related strains were correct. Both of positive samples and negative samples were uniform and stable. The satisfaction rates of assessment result were 97.1%, 94.7% and 100% respectively in 2018-2020.ConclusionThe samples could meet the requirements of proficiency test. By organization proficiency test, the difference of laboratories can be reflected which is helpful to further improve the laboratory test capability.http://www.zgspws.com/zgspwszz/article/abstract/20220106?st=article_issuecronobacter spp.proficiency testsample preparationassessment |
| spellingShingle | LIU Na ZHAO Linna WANG Xueshuo CUI Shenghui Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020 Zhongguo shipin weisheng zazhi cronobacter spp. proficiency test sample preparation assessment |
| title | Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020 |
| title_full | Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020 |
| title_fullStr | Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020 |
| title_full_unstemmed | Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020 |
| title_short | Preparation of quality control samples of Cronobacter spp. and their application in the proficiency test in 2018-2020 |
| title_sort | preparation of quality control samples of cronobacter spp and their application in the proficiency test in 2018 2020 |
| topic | cronobacter spp. proficiency test sample preparation assessment |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20220106?st=article_issue |
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