Ultra-rapid somatic variant detection via real-time targeted amplicon sequencing
A method for detection of somatic variants is presented for the intraoperative evaluation of tumor mutations in less than 30 min, using rapid DNA extraction, in silico optimized LAMP amplification, and a LAMP product analysis tool: LAMPrey.
Main Authors: | Jack Wadden, Brandon S. Newell, Joshua Bugbee, Vishal John, Amy K. Bruzek, Robert P. Dickson, Carl Koschmann, David Blaauw, Satish Narayanasamy, Reetuparna Das |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2022-07-01
|
Series: | Communications Biology |
Online Access: | https://doi.org/10.1038/s42003-022-03657-6 |
Similar Items
-
nPoRe: n-polymer realigner for improved pileup-based variant calling
by: Tim Dunn, et al.
Published: (2023-03-01) -
Cell-Free Tumor DNA (cf-tDNA) Liquid Biopsy: Current Methods and Use in Brain Tumor Immunotherapy
by: Jack Wadden, et al.
Published: (2022-04-01) -
Liquid biopsy in pediatric brain tumors
by: Arushi Tripathy, et al.
Published: (2023-01-01) -
Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection
by: Deepak Thirunavukarasu, et al.
Published: (2021-09-01) -
Allelic selection of amplicons in glioblastoma revealed by combining somatic and germline analysis.
by: Thomas LaFramboise, et al.
Published: (2010-09-01)