Microfluidic system for in vitro epithelial folding and calcium waves induction

Microfluidic system for in vitro epithelial folding and calcium waves induction

Summary: Epithelial folding is a fundamental process where initially flat monolayers transform into functional 3D structures. This protocol details fabrication steps for a polycarbonate microfluidic platform which enables triggering epithelial folds that recapitulate stereotypical cell shape changes...

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Bibliographic Details
Main Authors: Marvin Brun-Cosme-Bruny, Lydia Pernet, Slawomir Blonski, Damian Zaremba, Sandrine Fraboulet, Monika Elzbieta Dolega
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Cell culture
Cell isolation
Single cell
Developmental biology
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005639

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http://www.sciencedirect.com/science/article/pii/S2666166722005639

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