Alternative preparation of inclusion bodies excludes interfering non-protein contaminants and improves the yield of recombinant proinsulin
The goal of simple, high-yield expression and purification of recombinant human proinsulin has proven to be a considerable challenge. First, proinsulin forms inclusion bodies during bacterial expression. While this phenomenon can be exploited as a capture step, conventionally prepared inclusion bodi...
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| Format: | Article |
| Jezik: | English |
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Elsevier
2014-01-01
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| Serija: | MethodsX |
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| Online dostop: | http://www.sciencedirect.com/science/article/pii/S2215016114200483 |