Analysis of SHIP1 expression and activity in Crohn's disease patients.
SH2 domain containing inositol-5-phosphatase (SHIP1) is an important modulator of innate and adaptive immunity. In mice, loss of SHIP1 provokes severe ileitis resembling Crohn's disease (CD), as a result of deregulated immune responses, altered cytokine production and intestinal fibrosis. Recen...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5540589?pdf=render |
| _version_ | 1818683410879610880 |
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| author | Rajesh Somasundaram Sandra Fernandes Jasper J Deuring Colin de Haar Ernst J Kuipers Lauran Vogelaar Frank A Middleton C Janneke van der Woude Maikel P Peppelenbosch William G Kerr Gwenny M Fuhler |
| author_facet | Rajesh Somasundaram Sandra Fernandes Jasper J Deuring Colin de Haar Ernst J Kuipers Lauran Vogelaar Frank A Middleton C Janneke van der Woude Maikel P Peppelenbosch William G Kerr Gwenny M Fuhler |
| author_sort | Rajesh Somasundaram |
| collection | DOAJ |
| description | SH2 domain containing inositol-5-phosphatase (SHIP1) is an important modulator of innate and adaptive immunity. In mice, loss of SHIP1 provokes severe ileitis resembling Crohn's disease (CD), as a result of deregulated immune responses, altered cytokine production and intestinal fibrosis. Recently, SHIP1 activity was shown to be correlated to the presence of a CD-associated single nucleotide polymorphism in ATG16L1. Here, we studied SHIP1 activity and expression in an adult cohort of CD patients.SHIP1 activity, protein and mRNA expression in peripheral blood mononuclear cells from CD patients in clinical remission were determined by Malachite green assay, Western blotting and qRT-PCR respectively. Genomic DNA was genotyped for ATG16L1 rs2241880.SHIP1 protein levels are profoundly diminished in a subset of patients; however, SHIP1 activity and expression are not correlated to ATG16L1 SNP status in this adult cohort.Aberrant SHIP1 activity can contribute to disease in a subset of adult CD patients, and warrants further investigation. |
| first_indexed | 2024-12-17T10:34:18Z |
| format | Article |
| id | doaj.art-438601c795fd434ca47b09f3b310e90a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T10:34:18Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-438601c795fd434ca47b09f3b310e90a2022-12-21T21:52:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018230810.1371/journal.pone.0182308Analysis of SHIP1 expression and activity in Crohn's disease patients.Rajesh SomasundaramSandra FernandesJasper J DeuringColin de HaarErnst J KuipersLauran VogelaarFrank A MiddletonC Janneke van der WoudeMaikel P PeppelenboschWilliam G KerrGwenny M FuhlerSH2 domain containing inositol-5-phosphatase (SHIP1) is an important modulator of innate and adaptive immunity. In mice, loss of SHIP1 provokes severe ileitis resembling Crohn's disease (CD), as a result of deregulated immune responses, altered cytokine production and intestinal fibrosis. Recently, SHIP1 activity was shown to be correlated to the presence of a CD-associated single nucleotide polymorphism in ATG16L1. Here, we studied SHIP1 activity and expression in an adult cohort of CD patients.SHIP1 activity, protein and mRNA expression in peripheral blood mononuclear cells from CD patients in clinical remission were determined by Malachite green assay, Western blotting and qRT-PCR respectively. Genomic DNA was genotyped for ATG16L1 rs2241880.SHIP1 protein levels are profoundly diminished in a subset of patients; however, SHIP1 activity and expression are not correlated to ATG16L1 SNP status in this adult cohort.Aberrant SHIP1 activity can contribute to disease in a subset of adult CD patients, and warrants further investigation.http://europepmc.org/articles/PMC5540589?pdf=render |
| spellingShingle | Rajesh Somasundaram Sandra Fernandes Jasper J Deuring Colin de Haar Ernst J Kuipers Lauran Vogelaar Frank A Middleton C Janneke van der Woude Maikel P Peppelenbosch William G Kerr Gwenny M Fuhler Analysis of SHIP1 expression and activity in Crohn's disease patients. PLoS ONE |
| title | Analysis of SHIP1 expression and activity in Crohn's disease patients. |
| title_full | Analysis of SHIP1 expression and activity in Crohn's disease patients. |
| title_fullStr | Analysis of SHIP1 expression and activity in Crohn's disease patients. |
| title_full_unstemmed | Analysis of SHIP1 expression and activity in Crohn's disease patients. |
| title_short | Analysis of SHIP1 expression and activity in Crohn's disease patients. |
| title_sort | analysis of ship1 expression and activity in crohn s disease patients |
| url | http://europepmc.org/articles/PMC5540589?pdf=render |
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