A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants

A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants

Abstract Background The CRISPR/Cas9 system is being used for genome editing purposes by many research groups in multiple plant species. Traditional sequencing methods to identify homozygous mutants are time-consuming, laborious and expensive. Results We have developed a method to screen CRISPR/Cas9-...

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Bibliographic Details
Main Authors: Jinggong Guo, Kun Li, Lifeng Jin, Rui Xu, Kaiting Miao, Fengbo Yang, Chaoya Qi, Lin Zhang, Jose R. Botella, Ran Wang, Yuchen Miao
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Plant Methods
Subjects:
CRISPR/Cas9
Genome editing
Tobacco
Arabidopsis thaliana
PCR
Mutant screening
Online Access:http://link.springer.com/article/10.1186/s13007-018-0305-8

Internet

http://link.springer.com/article/10.1186/s13007-018-0305-8

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