Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models
In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson’s disease (PD). We identified ASYN in oligodendrocyte lineage progenit...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-08-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671115001940 |
| _version_ | 1818999254717300736 |
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| author | Mehdi Djelloul Staffan Holmqvist Antonio Boza-Serrano Carla Azevedo Maggie S. Yeung Stefano Goldwurm Jonas Frisén Tomas Deierborg Laurent Roybon |
| author_facet | Mehdi Djelloul Staffan Holmqvist Antonio Boza-Serrano Carla Azevedo Maggie S. Yeung Stefano Goldwurm Jonas Frisén Tomas Deierborg Laurent Roybon |
| author_sort | Mehdi Djelloul |
| collection | DOAJ |
| description | In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson’s disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a+ cells and SOX10+ oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease. |
| first_indexed | 2024-12-20T22:14:30Z |
| format | Article |
| id | doaj.art-43b678cb6e484222a04d5d12aa1b7630 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-20T22:14:30Z |
| publishDate | 2015-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-43b678cb6e484222a04d5d12aa1b76302022-12-21T19:25:05ZengElsevierStem Cell Reports2213-67112015-08-015217418410.1016/j.stemcr.2015.07.002Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human ModelsMehdi Djelloul0Staffan Holmqvist1Antonio Boza-Serrano2Carla Azevedo3Maggie S. Yeung4Stefano Goldwurm5Jonas Frisén6Tomas Deierborg7Laurent Roybon8Stem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, SwedenStem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, SwedenStrategic Research Area MultiPark, Lund University, 22184 Lund, SwedenStem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, SwedenDepartment of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, SwedenParkinson Institute, Istituti Clinici di Perfezionamento, 20126 Milan, ItalyDepartment of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, SwedenStrategic Research Area MultiPark, Lund University, 22184 Lund, SwedenStem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, SwedenIn this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson’s disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a+ cells and SOX10+ oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.http://www.sciencedirect.com/science/article/pii/S2213671115001940 |
| spellingShingle | Mehdi Djelloul Staffan Holmqvist Antonio Boza-Serrano Carla Azevedo Maggie S. Yeung Stefano Goldwurm Jonas Frisén Tomas Deierborg Laurent Roybon Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models Stem Cell Reports |
| title | Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models |
| title_full | Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models |
| title_fullStr | Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models |
| title_full_unstemmed | Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models |
| title_short | Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models |
| title_sort | alpha synuclein expression in the oligodendrocyte lineage an in vitro and in vivo study using rodent and human models |
| url | http://www.sciencedirect.com/science/article/pii/S2213671115001940 |
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