Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein
Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the trans-splicing of CPE...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
China Science Publishing & Media Ltd.
2023-07-01
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Series: | Acta Biochimica et Biophysica Sinica |
Subjects: | |
Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2023135 |