Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein

Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the trans-splicing of CPE...

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Bibliographic Details
Main Authors: Zhan Qin, Shi Changhua, Jiang Yu, Gao Xianling, Lin Ying
Format: Article
Language:English
Published: China Science Publishing & Media Ltd. 2023-07-01
Series:Acta Biochimica et Biophysica Sinica
Subjects:
Online Access:https://www.sciengine.com/doi/10.3724/abbs.2023135