Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein

Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein

Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the trans-splicing of CPE...

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Bibliographic Details
Main Authors: Zhan Qin, Shi Changhua, Jiang Yu, Gao Xianling, Lin Ying
Format: Article
Language:English
Published: China Science Publishing & Media Ltd. 2023-07-01
Series:Acta Biochimica et Biophysica Sinica
Subjects:
<italic>Cne</italic> PRP8 intein
C-extein
directed evolution
Online Access:https://www.sciengine.com/doi/10.3724/abbs.2023135

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