Xylanase gene mutation by error-prone pcr and expression in Pichia pastoris

Xylanase gene mutation by error-prone pcr and expression in Pichia pastoris

Xylanase can hydrolyze xylan for reducing its anti-nutritional impact and improving nutrient availability, so obtaining suitable xylanase to degrade xylan is essential. Error-prone PCR and gene transformation were used in this study to obtain the ideal xylanase for degrading xylan effectively. The r...

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Bibliographic Details
Main Authors: Shuli Li, Weiwei Huang, Juan Chang, Ping Wang, Qingqiang Yin, Chaoqi Liu, Erzhu Wang, Fushan Lu
Format: Article
Language:English
Published: Universidade Federal de Uberlândia 2018-05-01
Series:Bioscience Journal
Subjects:
error-prone pcr
xylanase
gene mutation
gene expression
pichia pastoris
Online Access:http://www.seer.ufu.br/index.php/biosciencejournal/article/view/38530

Internet

http://www.seer.ufu.br/index.php/biosciencejournal/article/view/38530

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