Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella)
This study attempted to explore the adaptability of gut microbiota in grass carp to diets with different carbohydrate-to-protein ratios in order to better understand the immunological and metabolic changes in the organism. Three different ratios of carbohydrate and protein diets (12.5%-carbohydrate...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002818 |
| _version_ | 1797676611560013824 |
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| author | Yanpeng Zhang Manjie Sun Yucheng Liu Ting Chu Xianjie Liu Zhihao Cui Shengzhen Jin Xiaochen Yuan |
| author_facet | Yanpeng Zhang Manjie Sun Yucheng Liu Ting Chu Xianjie Liu Zhihao Cui Shengzhen Jin Xiaochen Yuan |
| author_sort | Yanpeng Zhang |
| collection | DOAJ |
| description | This study attempted to explore the adaptability of gut microbiota in grass carp to diets with different carbohydrate-to-protein ratios in order to better understand the immunological and metabolic changes in the organism. Three different ratios of carbohydrate and protein diets (12.5%-carbohydrate 46.3%-protein (LCHP), 27.5%-carbohydrate 33.7%-protein (MCMP), and 42.5%-carbohydrate 16.9%-protein (HCLP)) were satiety-fed to grass carp. After feeding, the growth performance, gut microbial community composition and function were analyzed. Growth performance results showed that LCHP group exhibited the significantly lower specific growth ratio than MCMP group, and HCLP group displayed the significantly higher feed coefficient and lower survival rate. The community composition results showed that the abundance of the beneficial genus Cetobacterium was the highest in MCMP group, and the abundance of the harmful genera Ruminiclostridium_9 and Phreatobacter was the highest in the LCHP and HCLP groups, respectively. Furthermore, the ratio of Firmicutes to Bacteroidetes considered as an indicator of gut microbiota dysfunction, was significantly higher in the LCHP and HCLP groups than that in the MCMP group. The community functional analysis showed that the abundance levels of cofactors and vitamin metabolism were significantly lower and the abundance levels of neurodegenerative diseases were significantly higher in the LCHP and HCLP groups than those in the MCMP group. The abundance levels of energy metabolism and a range of biomolecules metabolism were significantly decreased in the HCLP group. These results suggested that high-carbohydrate diets have a greater negative impact on the gut microbiota than high-protein diets. The gut microbiota results echoed the growth performance parameters, reflecting that gut microbiota may play an important role in the influence of different carbohydrate-to-protein ratios diets on host. Bacterial genera such as Ruminiclostridium_9 and Phreatobacter may play key roles in the effects of high-protein and high-carbohydrate diets on host health and metabolism, respectively. |
| first_indexed | 2024-03-11T22:31:44Z |
| format | Article |
| id | doaj.art-442712d395d642e082745a4615b3222f |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-11T22:31:44Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-442712d395d642e082745a4615b3222f2023-09-23T05:11:32ZengElsevierAquaculture Reports2352-51342023-10-0132101742Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella)Yanpeng Zhang0Manjie Sun1Yucheng Liu2Ting Chu3Xianjie Liu4Zhihao Cui5Shengzhen Jin6Xiaochen Yuan7College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaCorrespondence to: College of Animal Science and Technology, Anhui Agricultural University, No. 130, Changjiang Road, Shushan District, Hefei, Anhui Province 230036, China.; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, ChinaThis study attempted to explore the adaptability of gut microbiota in grass carp to diets with different carbohydrate-to-protein ratios in order to better understand the immunological and metabolic changes in the organism. Three different ratios of carbohydrate and protein diets (12.5%-carbohydrate 46.3%-protein (LCHP), 27.5%-carbohydrate 33.7%-protein (MCMP), and 42.5%-carbohydrate 16.9%-protein (HCLP)) were satiety-fed to grass carp. After feeding, the growth performance, gut microbial community composition and function were analyzed. Growth performance results showed that LCHP group exhibited the significantly lower specific growth ratio than MCMP group, and HCLP group displayed the significantly higher feed coefficient and lower survival rate. The community composition results showed that the abundance of the beneficial genus Cetobacterium was the highest in MCMP group, and the abundance of the harmful genera Ruminiclostridium_9 and Phreatobacter was the highest in the LCHP and HCLP groups, respectively. Furthermore, the ratio of Firmicutes to Bacteroidetes considered as an indicator of gut microbiota dysfunction, was significantly higher in the LCHP and HCLP groups than that in the MCMP group. The community functional analysis showed that the abundance levels of cofactors and vitamin metabolism were significantly lower and the abundance levels of neurodegenerative diseases were significantly higher in the LCHP and HCLP groups than those in the MCMP group. The abundance levels of energy metabolism and a range of biomolecules metabolism were significantly decreased in the HCLP group. These results suggested that high-carbohydrate diets have a greater negative impact on the gut microbiota than high-protein diets. The gut microbiota results echoed the growth performance parameters, reflecting that gut microbiota may play an important role in the influence of different carbohydrate-to-protein ratios diets on host. Bacterial genera such as Ruminiclostridium_9 and Phreatobacter may play key roles in the effects of high-protein and high-carbohydrate diets on host health and metabolism, respectively.http://www.sciencedirect.com/science/article/pii/S2352513423002818Core genusCommunity compositionHigh-carbohydrate/protein dietsHost health and metabolismMicroecological function |
| spellingShingle | Yanpeng Zhang Manjie Sun Yucheng Liu Ting Chu Xianjie Liu Zhihao Cui Shengzhen Jin Xiaochen Yuan Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella) Aquaculture Reports Core genus Community composition High-carbohydrate/protein diets Host health and metabolism Microecological function |
| title | Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella) |
| title_full | Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella) |
| title_fullStr | Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella) |
| title_full_unstemmed | Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella) |
| title_short | Gut microbiota adaptation to low and high carbohydrate-to-protein ratio diets in grass carp (Ctenopharyngodon idella) |
| title_sort | gut microbiota adaptation to low and high carbohydrate to protein ratio diets in grass carp ctenopharyngodon idella |
| topic | Core genus Community composition High-carbohydrate/protein diets Host health and metabolism Microecological function |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002818 |
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